Evidence map›Paper›PMID 41838124›Full record

ArticlePediatric surgery international2026

Profiling circular RNAs in amniotic fluid and fetal lungs from congenital diaphragmatic hernia cases: insights into potential prognostic and diagnostic applications.

Nolan S De Leon, Arzu Öztürk, Francesca M Russo, Wai Hei Tse, Claire McCallum, Benjamin Eyrikson, Jodi Qiao, Daywin Patel, Richard LeDuc, Jan A Deprest and 1 more

Abstract read
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In one paragraph

Article in Pediatric surgery international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nolan S De LeonDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Arzu ÖztürkDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Francesca M RussoClinical Department of Obstetrics and Gynaecology, University Hospitals Leuven and Academic Department Development and Regeneration, Organ System Cluster, KU Leuven, Leuven, Belgium.
Wai Hei TseDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Claire McCallumDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Benjamin EyriksonDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Jodi QiaoDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Daywin PatelDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Richard LeDucDepartment of Biochemistry and Medical Genetics, University of Manitoba and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Jan A DeprestClinical Department of Obstetrics and Gynaecology, University Hospitals Leuven and Academic Department Development and Regeneration, Organ System Cluster, KU Leuven, Leuven, Belgium.
Richard KeijzerDepartment of Surgery, Division of Pediatric Surgery, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, and Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada. rkeijzer@hsc.mb.ca.

Funding

CIHR 178347
6 · The paper itself

Abstract

purposeCircular RNAs (circRNAs), generated by backsplicing, are stable closed-loop RNAs. CircRNAs in amniotic fluid (AF) may serve as diagnostic and prognostic markers for Congenital Diaphragmatic Hernia (CDH). This study profiled AF circRNAs from CDH pregnancies to explore associations with CDH pathways and survival.

methodsAF was collected from CDH pregnancies undergoing fetoscopic endoluminal tracheal occlusion (FETO) (n = 8; 4 survivors, 4 non-survivors). CircRNAs were profiled using the Arraystar Human circRNA Array V2. Differential expression was analyzed with limma in R and compared to our previously published data from CDH lungs. Parental genes underwent gene ontology and KEGG analyses, and selected circRNAs were validated by sequencing of backsplice junctions.

resultsA total of 10,760 circRNAs were detected in AF, 12,055 in mid-gestation lung, and 10,118 in late-gestation lung. In AF, 514 circRNAs were differentially expressed (False Discovery Rate (FDR) < 0.05) with overlapping circRNAs, including 24 with late-gestation lung and 4 with CDH-related genes. CircFOXP2 (12-19) and circSMARCC1 (16-14) were validated. Parental genes of these differentially expressed AF circRNAs were enriched in specific molecular and signaling pathways.

conclusionsAF circRNAs distinguish CDH survivors from non-survivors, mapping to genes and pathways critical for development, suggesting their diagnostic and prognostic potential.

Indexed as

Amniotic FluidHernias, Diaphragmatic, CongenitalLungRNA, CircularBiomarkersFemaleFetoscopyHumansPregnancyPrognosisBiomarkersRNA, CircularBiomarkerCircular RNACongenital diaphragmatic herniaEpigenetic

Identifiers

PMID41838124

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.