Real-time polymerase chain reaction results of urogenital system samples and their relationship with other sexually transmitted diseases.

Objectives: This study aimed to examine the results obtained by multiplex real-time polymerase chain reaction (PCR) for microorganisms in urogenital tract samples sent to the molecular microbiology laboratory than patients of sexually transmitted infections suspected. In addition, we evaluated them alongside HIV, HBV, HCV, and syphilis test results. Method: Urine, vaginal swab, and urethral discharge samples submitted to Duzce University, Medical Microbiology Laboratory between March 2023 to November 2023 were included in the cross-sectional study. Multiplex PCR was used to assess the positivity of Results: A total of one hundred patient samples were sent to the Molecular Microbiology Laboratory during the study period. Twenty-eight patients were female and 72 were male, and the mean age was 34.11±10.63 (19-72). A total of 109 positive pathogens were detected in 65 (65%) patients. The positivity rate in women was 75% and in men was 62%, and was statistically similar (p=0.191). The most common pathogen detected in women was Conclusion: Molecular microbiological methods may be useful in patients suspected of having a urogenital system infection when no pathogens are cultured or when the presence of multiple pathogens must be identified to guide treatment.