Evidence map›Paper›PMID 41835007›Full record

ArticleFrontiers in cellular and infection microbiology2026

Development of a novel duplex crystal digital PCR for the detection of PRRSV-1 and PRRSV-2.

Yuwen Shi, Jiakang He, Kaichuang Shi, Yanwen Yin, Feng Long, Shuping Feng, Zuzhang Wei

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yuwen Shi *Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Jiakang He *Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Kaichuang ShiGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Yanwen YinGuangxi Center for Animal Disease Control and Prevention, Nanning, China.
Feng LongGuangxi Center for Animal Disease Control and Prevention, Nanning, China.
Shuping FengGuangxi Center for Animal Disease Control and Prevention, Nanning, China.
Zuzhang WeiGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Porcine reproductive and respiratory syndrome (PRRS) is a widely prevalent disease of reproductive failure of pregnant pigs and respiratory syndromes in pigs of different ages, especially in piglets. The etiological agents include PRRS virus (PRRSV) genotypes 1 (PRRSV-1) and PRRSV-2, whereas their clinical symptoms are similar and hard to differentiate. It is necessary to establish accurate and reliable methods for differential detection of PRRSV-1 and PRRSV-2. Methods: Two pairs of specific primers and probes were designed basing on the PRRSV-1 and PRRSV-2 ORF6 gene. The reaction conditions and procedures of the duplex crystal digital PCR (cdPCR) were optimized. The specificity, sensitivity, and repeatability of the developed assay were evaluated. The application of the developed assay was assessed by testing 2,185 clinical tissue samples. Results: The results indicated that the concentration of the templates and their Ct values had good linear relationship with R Conclusions: These results indicated that a rapid and accurate duplex cdPCR method with high sensitivity and excellent specificity had been successfully developed for the differential detection of PRRSV-1 and PRRSV-2.

Indexed as

Polymerase Chain ReactionPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusAnimalsDNA PrimersGenotypeReproducibility of ResultsSensitivity and SpecificitySwineDNA Primerscrystal digital PCR (cdPCR)differential detectionporcine reproductive and respiratory syndrome virus (PRRSV)PRRSV–2PRRSV genotype 1(PRRSV–1)

Identifiers

PMID41835007
PMCPMC12982388

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