ArticleFrontiers in veterinary science2026
Establishment and field validation of a rapid on-site recombinase polymerase amplification-lateral flow assay for BRSV and BVDV.
Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine respiratory disease (BRD) is the costliest bovine syndrome worldwide, inflicting annual losses of over one billion USD in North America alone. Transport stress, overcrowding and viral-bacterial synergy can drive mortality to 70%, yet laboratory-based diagnostics delay decisive treatment. We therefore developed pen-side real-time enzymatic recombinase amplification lateral-flow dipsticks (RT-ERA-LFD) assays targeting the two principal viral agents, bovine respiratory syncytial virus (BRSV) and bovine viral diarrhoea virus (BVDV), which enables their separate detection in a single tube. The BRSV nucleoprotein gene and BVDV 5'-UTR were cloned and in-vitro transcribed into quantified RNA standards to calibrate an enzymatic recombinase amplification (ERA) coupled with lateral-flow dipsticks (LFD). After primer/probe optimisation (BRSV-ERA-F1/R4/P2; BVDV-ERA-F1/R4/P1), the 40 °C, 20-min reactions detected as few as 10 template copies, showed 100% specificity against related bovine pathogens and matched real-time PCR results in 46 archived respiratory samples. In a field survey of nasal swabs from cattle farms in Jilin Province, China, BRSV was detected in 10.87% and BVDV in 8.70% of specimens, with results identical to qPCR obtained within 30 min without instrumentation. By delivering actionable infection status at the chute, the platform enables mass screening and timely intervention, effectively mitigating BRD's global economic impact.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.