Evidence map›Paper›PMID 41834322›Full record

ArticleJournal of fish diseases2026

Development and Validation of a Viability RT-qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus (SVCV).

Ha-Jeong Son, Min-Young Sohn, Jae-Ok Kim, Hee Jung Choi, Mun-Gyeong Kwon, Jeong-Tae Lee, Gyoungsik Kang, HyeongJin Roh, Chan-Il Park, Kyung-Ho Kim

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fish diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ha-Jeong SonDepartment of Marine Biology and Aquaculture, College of Marine Sciences, Gyeongsang National University, Tongyeong, Republic of Korea.
Min-Young SohnDepartment of Marine Biology and Aquaculture, College of Marine Sciences, Gyeongsang National University, Tongyeong, Republic of Korea.
Jae-Ok KimAquatic Disease Control Division, National Fishery Products Quality Management Service, Busan, Republic of Korea.
Hee Jung ChoiAquatic Disease Control Division, National Fishery Products Quality Management Service, Busan, Republic of Korea.
Mun-Gyeong KwonAquatic Disease Control Division, National Fishery Products Quality Management Service, Busan, Republic of Korea.
Jeong-Tae LeeGyeongsangnam-Do Fisheries Safety Technology Institute, Tongyeong, Republic of Korea.
Gyoungsik KangDepartment of Aquatic Life Medicine, College of Marine Sciences, Gyeongsang National University, Tongyeong, Republic of Korea.ORCID https://orcid.org/0000-0001-9160-6371
HyeongJin RohDepartment of Aquatic Life Medicine, College of Marine Sciences, Gyeongsang National University, Tongyeong, Republic of Korea.
Chan-Il ParkDepartment of Marine Biology and Aquaculture, College of Marine Sciences, Gyeongsang National University, Tongyeong, Republic of Korea.
Kyung-Ho KimDepartment of Aquatic Life Medicine, College of Marine Sciences, Gyeongsang National University, Tongyeong, Republic of Korea.

Funding

National Fishery Products Quality Management Service NFQS2026001
6 · The paper itself

Abstract

Spring viremia of carp virus (SVCV) is a contagious pathogen associated with significant mortality and economic losses in freshwater aquaculture. Although reverse transcription quantitative PCR (RT-qPCR) enables rapid detection, it does not distinguish between infectious and non-infectious viral particles, which may lead to overestimation of infection risk. In this study, a viability RT-qPCR (vqPCR) assay targeting the glycoprotein (G) gene of SVCV was developed and analytically evaluated. Primers and probes were designed based on 45 full-length G-gene sequences representing genogroups Ia-Id to ensure broad genetic coverage. The assay achieved a 95% limit of detection (LoD

Indexed as

Fish DiseasesReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRhabdoviridaeRhabdoviridae InfectionsAnimalsAquacultureCarpsReproducibility of ResultsRNA, ViralSensitivity and SpecificityRNA, Viralaquatic virusdiagnostic performanceinfectivityquantitative PCR assayviability PCR

Identifiers

PMID41834322
PMCPMC13331534

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.