Evidence map›Paper›PMID 41833377›Full record

ArticleThe Journal of veterinary medical science2026

Re-maturation of canine chemically-induced hepatic progenitor cells toward biliary epithelial cells.

Kaoruko Kikuchi, Kotaro Nishi, Yoko Yamada, Yu Yamazaki, Sakurako Neo, Toshihiro Tsukui, Satoko Tateishi, Hirotaka Igarashi, Akihide Kamiya, Masaharu Hisasue

Abstract read
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In one paragraph

Article in The Journal of veterinary medical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kaoruko KikuchiLaboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Kotaro NishiLaboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Yoko YamadaLaboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Yu YamazakiLaboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Sakurako NeoLaboratory of Clinical Diagnostics, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Toshihiro TsukuiNihon Zenyaku Co., Ltd., Fukushima, Japan.
Satoko TateishiNihon Zenyaku Co., Ltd., Fukushima, Japan.
Hirotaka IgarashiLaboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Akihide KamiyaDepartment of Molecular Life Sciences, Tokai University School of Medicine, Kanagawa, Japan.
Masaharu HisasueLaboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to evaluate the differentiation potential of canine hepatic progenitor cells (cHPCs) derived from cryopreserved hepatocytes (cHeps). The cHeps were reprogrammed using three small molecules, Y-27632, A-83-01, and CHIR99021, to establish cHPCs. The cells were cultured on Matrigel in a chemically defined feeder-free maintenance medium for human pluripotent stem cells containing YAC, followed by 2% growth factor-reduced Matrigel. During culture, cHPCs transitioned from two-dimensional sheets to three-dimensional ductal structures. qRT-PCR and immunohistochemistry revealed increased CK19 expression on the ductal surfaces, indicating biliary epithelial-like differentiation. Overall, cHPCs possess a bipotential rematuration capacity. This study demonstrated that cHPCs induced by the YAC cocktail possess bipotent differentiation capabilities, confirming that their characteristics are likely to chemically induce liver progenitor cells.

Indexed as

Cell DifferentiationEpithelial CellsHepatocytesStem CellsAmidesAnimalsCell Culture TechniquesCells, CulturedCollagenCryopreservationDogsDrug CombinationsLamininProteoglycansPyridinesPyrimidinesAmidesChir 99021CollagenDrug CombinationsLamininmatrigelProteoglycansPyridinesPyrimidinesY 27632biliary epithelial cellcanine hepatic progenitorchemically-induced liver progenitor cellhepatocyte maturationin vitro liver model

Identifiers

PMID41833377
PMCPMC13280256

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.