Protocol for targeted peripheral adeno-associated viral vector transduction of the intradental neurons in mice.

Ling-Yu Liu, Amy Chang, Isaac T Berthaume, Joshua J Emrick

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ling-Yu LiuDepartment of Biologic and Materials Sciences & Prosthodontics, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: liuly@umich.edu.

Amy ChangDepartment of Biologic and Materials Sciences & Prosthodontics, University of Michigan, Ann Arbor, MI 48109, USA.

Isaac T BerthaumeDepartment of Biologic and Materials Sciences & Prosthodontics, University of Michigan, Ann Arbor, MI 48109, USA.

Joshua J EmrickDepartment of Biologic and Materials Sciences & Prosthodontics, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: jjemrick@umich.edu.

Funding

Determining the functions of tooth-innervating neurons in dental sensationK22DE029779 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI EMRICK, JOSHUA JAMES · 2022 to 2024

$742k

NIDCR NIH HHS K22 DE029779

6 · The paper itself

Abstract

Here, we present the methodology for surgical preparation of the mouse molar teeth and subsequent adeno-associated virus (AAV) transduction of intradental neurons within the tooth (AAV-i.d.). We describe steps for mouse positioning, microscope setup, drilling to expose pulp horns, and applying the AAV-silk mixture. We then detail procedures for composite placement to cover the preparation and trigeminal ganglion (TG) screening to visualize the surface and assess and verify AAV labeling. AAV-i.d. labels intradental neurons and traces their associated neuronal projections. For complete details on the use and execution of this protocol, please refer to Ronan et al.

Indexed as

DependovirusGenetic VectorsNeuronsTransduction, GeneticAnimalsMiceMolarTrigeminal Ganglionhealth sciencesmodel organismsmolecular/chemical probesneuroscience

Identifiers

PMID41832951

DOI10.1016/j.xpro.2026.104424

PMCPMC13000510

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.