ArticleVirology journal2026
Development of reference material from Rift Valley fever virus RNA.
Article in Virology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors.
Funding
Abstract
backgroundRift Valley fever virus (RVFV) has mainly been reported in African countries, and molecular diagnosis is performed using nucleic acid amplification tests. Nucleic acid reference materials (RMs) are necessary for the performance evaluation and quality assessment of molecular diagnosis of Rift Valley fever. However, metrological certified RMs for RVFV nucleic acids are currently unavailable.
methodsIn this study, RVFV RNA RMs (KRISS 111-10-537, 538, and 539) were developed from in vitro transcribed RNA containing a three-segment genome, including the L, M, and S segments. The RNA copy numbers of the L, M, and S segments of RMs were measured using reverse-transcription droplet digital PCR (RT-ddPCR), an advanced technology that enables the absolute quantification of viral genetic material.
resultsIn the homogeneity analysis, the copy number values were measured to be from 7.82 × 10
conclusionsRMs can help reduce false positives and negatives in RVFV molecular tests by providing accurate reference values for target viral gene copy numbers, ultimately contributing to better diagnosis and control of this viral disease.
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