Evidence map›Paper›PMID 41832242›Full record

ArticleScientific reports2026

SMC ensures efficient chromosome replication and oriC positioning during Streptomyces spore germination.

Katarzyna Pawlikiewicz, Agnieszka Strzałka, Agnieszka Nurek, Magdalena Donczew, Anna Gierlikowska, Martyna Gongerowska-Jac, Marcin J Szafran, Dagmara Jakimowicz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Katarzyna PawlikiewiczDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Agnieszka StrzałkaDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Agnieszka NurekDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Magdalena DonczewDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Anna GierlikowskaDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Martyna Gongerowska-JacDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Marcin J SzafranDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
Dagmara JakimowiczDepartment of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland. dagmara.jakimowicz@uwr.edu.pl.

Funding

Narodowe Centrum Nauki OPUS grant 2018/31/B/NZ1/00614
6 · The paper itself

Abstract

Bacterial chromosomes are organized by condensins, such as Structural Maintenance of Chromosomes (SMC) proteins. In Streptomyces, a genus of sporulating bacteria, SMC proteins align chromosomal arms and promote efficient compaction of chromosomal DNA during spore formation. We hypothesized that disrupting nucleoid architecture by deleting the smc gene would affect the positioning of the origin of replication (oriC) or the process of chromosome replication during spore germination. To test this hypothesis, we conducted marker frequency analyses and microscopy studies to observe the positioning of labelled oriC and replisomes in both wild type and Δsmc backgrounds. Additionally, we investigated the positioning of three chromosomal loci in early vegetative cells. Our results indicate that the deletion of smc impairs chromosome replication and hinders germ tube development. Furthermore, detailed analysis of chromosome organization revealed that, in the absence of SMC, the oriC region becomes mispositioned within the nucleoid. These findings underscore the important role of SMC in maintaining nucleoid architecture during the early growth stages of Streptomyces.

Indexed as

Bacterial ProteinsCell Cycle ProteinsChromosomes, BacterialDNA ReplicationOrigin Recognition ComplexReplication OriginSpores, BacterialStreptomycesDNA, BacterialBacterial ProteinsCell Cycle ProteinsDNA, BacterialOriC chromosomal replication originOrigin Recognition ComplexSMC protein, BacteriaBacterial chromosome organizationChromosome replicationCondensinsSpore germination

Identifiers

PMID41832242
PMCPMC13121528

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.