Evidence map›Paper›PMID 41831262›Full record

ArticleUltrasonics sonochemistry2026

Sonication integrated with micronization: structural modification to improve the in-vitro digestibility, functional, and antioxidant properties of red pepper seed protein isolate.

Muhammad Faisal Manzoor, Muhammad Waseem, Tazeddinova Diana, Noman Walayat, Tosheva Zilolakhon Abduvalievna, Rana Muhammad Aadil, Shahzad Hussain, Zahoor Ahmed, Murtaza Ali, Xin-An Zeng and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ultrasonics sonochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Muhammad Faisal ManzoorGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, China. Electronic address: faisaluos26@gmail.com.
Muhammad WaseemCollege of Ocean Food and Biological Engineering, Jimei University, Xiamen, China.
Tazeddinova DianaDepartment of Technology and Organization of Public Catering, Institute of Sport Tourism and Service, South Ural State University, Chelyabinsk, Russian Federation.
Noman WalayatCollege of Tea Science and Tea Culture, Zhejiang Agriculture and Forestry University, Hangzhou, China.
Tosheva Zilolakhon AbduvalievnaDepartment of Technology and Organization of Public Catering, Institute of Sport Tourism and Service, South Ural State University, Chelyabinsk, Russian Federation.
Rana Muhammad AadilNational Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan.
Shahzad HussainDepartment of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, Saudi Arabia.
Zahoor AhmedSchool of Food and Agricultural Sciences, University of Management and Technology, Lahore, Pakistan.
Murtaza AliGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Xin-An ZengGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, China. Electronic address: xazeng@scut.edu.cn.
Abderrahmane Aït-KaddourUniversité Clermont Auvergne, INRAE, VetAgro Sup, UMRF, 63370 Lempdes, France. Electronic address: abderrahmane.aitkaddour@vetagro-sup.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The study aimed to extract protein from red pepper seeds and to probe the effects of micronization (MN) at 15,000 rpm for 3 and 6 min, ultrasound (US) at 720 W, 40 kHz for 10 and 15 min, and their combined impact on red pepper seed protein isolate (RPSPI). The combined treatment (MN 6 min and US 10 min) substantially reduced particle size and increased -ve zeta potential, thereby significantly increasing RPSPI stability compared to untreated and individual treatments. Structural results showed significant molecular changes: increased free-SH content and surface hydrophobicity, and decreased intrinsic fluorescence intensity, indicating improved exposure of buried residues and partial unfolding. Fourier Transform Infrared peak spectra results verified secondary structural modification, evidenced by a significant increase in random coil and α-helix content. The disruption and significant fragmentation observed in Scanning Electron Microscope micrographs confirmed these findings. Thermograms from Differential Scanning Calorimetry and X-ray diffraction patterns showed decreases in the thermal transition temperature and crystallinity, respectively, indicating weak intermolecular interactions. These structural changes in RPSPI significantly increased the water solubility, emulsifying activity, foaming capacity, and water and oil holding capacity, while decreasing the turbidity. Also, combined treatment significantly enhanced the ABTS, DPPH, and OH scavenging abilities (%) of RPSPI and in vitro protein digestibility. Conclusively, results revealed that this extremely underexploited by-product protein can develop into a value-added ingredient by improving its techno-functional and bioactive properties through these non-thermal technologies.

Indexed as

AntioxidantsCapsicumDigestionPlant ProteinsSeedsSonicationParticle SizeSolubilityAntioxidantsPlant ProteinsCombined treatmentMicronization and ultrasoundRed pepper seed proteinStructural modificationSustainable green processingTechno-functional properties

Identifiers

PMID41831262
PMCPMC13000716

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.