ArticleFoods (Basel, Switzerland)2026
The Impact of Varying Enzymatic Pretreatment Durations of Wheat Gluten on the Flavour Characteristics of High-Moisture Plant-Based Extrudates.
Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study examined the effects of varying enzymatic pretreatment durations (0-80 min) of wheat gluten on flavour characteristics of high-moisture plant-based extrudates (HMPEs). Through a comprehensive analysis involving sensory evaluation, electronic tongue, free amino acid (FAA) profiling, electronic nose, and headspace solid-phase microextraction-gas chromatography-mass spectrometer (HS-SPME-GC-MS) analysis of volatile odour compounds, it was found that HMPEs with moderate enzymatic pretreatment (40 min) achieved the highest overall sensory score. Electronic tongue and FAA results confirmed a significant enhancement in umami and sweetness, while electronic nose effectively discriminated differences in odour profiles. Extending pretreatment durations gradually reduced beany off-flavours substances (hexanal reduced by up to 174.7 μg/kg) and encouraged the formation of meaty aroma compounds (furans and pyrazines). However, excessive pretreatment (>40 min) reduced acceptance due to burnt odour caused by the excessive accumulation of pyrazines, particularly 2,3-diethyl-5-methylpyrazine. Six key volatile odour compounds were identified by integrating the analysis of variable importance projection (VIP ≥ 1) and relative odour activity value (ROAV ≥ 1), offering a foundation for targeted flavour regulation in HMPEs.
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