Application of Enzyme Engineering and Synthetic Biology for Modulated Transformation of Fructooligosaccharides (FOSs) to Elucidate the Catalytic Mechanism of Fructofuranosidases.

Gan-Lin Chen, Jing Chen, Ling-Zhi Zhao, Bo Lin, Feng-Jin Zheng, Krishan K Verma, Li-Fang Yang

Abstract read

In one paragraph

Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Gan-Lin ChenSchool of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning 530006, China.ORCID 0000-0003-0076-5268

Jing ChenGuangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530001, China.

Ling-Zhi ZhaoCollege of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.

Bo LinInstitute of Agro-Products Processing Science and Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.

Feng-Jin ZhengInstitute of Agro-Products Processing Science and Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.

Krishan K VermaSugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.ORCID 0000-0002-5501-7905

Li-Fang YangSchool of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning 530006, China.

Funding

earmarked fund for China Agriculture Research System Guangxi Innovation Team - Specialty Fruits nycytxgxcxtd-2024-17Guangxi Academy of Agricultural Sciences Basic Research Business Project GNK2026YT164Guangxi Science and Technology Program GK-AA22117015-3opening project of Guangxi Key Laboratory of Green Processing of Sugar Resources GXTZYKF202211

6 · The paper itself

Abstract

Fructooligosaccharides (FOSs) are plant-based prebiotics widely utilized in the food and pharmaceutical industries. As a major sugar-producing region, Guangxi holds significant potential for enzymatic production of FOS from sucrose. This study engineered a mutant enzyme, 142P-242K, to address the low catalytic activity characteristic of wild-type enzymes. The mutation upregulated the FOS conversion efficiency from 29 to 52%, respectively. Optimal enzymatic activity was observed at 45 °C, pH 6.0, and in the presence of 1 mM Na

Indexed as

1-kestoseenzyme engineeringfructofuranosidasesfructooligosaccharide (FOS)nystosesynthetic biology

Identifiers

PMID41829116

DOI10.3390/foods15050843

PMCPMC12985077

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.