ArticleApplied microbiology and biotechnology2026
Optimizing cryopreservation protocols of Saccharomyces eubayanus using heat transfer modeling.
Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Efficient cryopreservation is essential for maintaining the viability of Saccharomyces eubayanus, a cryotolerant wild yeast of industrial importance as the cold-adapted parent of Saccharomyces pastorianus. This study integrated post-thaw viability and vitality assessments of cryopreserved S. eubayanus CRUB 1568ᵀ with experimental measurements of transient heat transfer and numerical simulation of the freezing stage. Two protocols were evaluated: (A) direct freezing of cryovials in cryoboxes at - 80 °C, governed by convection, and (B) freezing inside a CoolCell® device (Corning Inc., Corning, NY, USA), where heat transfer occurs by conduction through the insulated plastic material. A mathematical model was developed to numerically solve the transient heat transfer equation with phase change using the finite element method. Experimental temperature-time data validated the simulations, allowing estimation of overall heat transfer coefficients (UA = 18.04 W m
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