Evidence map›Paper›PMID 41826305›Full record

ArticleNature communications2026

Non-invasive imaging of defence responses in plants.

Anastasia V Balakireva, Tatiana A Karataeva, Michael Karampelias, Tatiana Yu Mitiouchkina, Viktor V Morozov, Jan Macháček, Ekaterina S Shakhova, Maxim M Perfilov, Olga A Belozerova, Sergey I Kovalchuk and 19 more

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Anastasia V Balakireva *Planta LLC, Moscow, Russia.
Tatiana A Karataeva *Planta LLC, Moscow, Russia.
Michael Karampelias *Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.ORCID http://orcid.org/0000-0002-2095-988X
Tatiana Yu Mitiouchkina *Planta LLC, Moscow, Russia.
Viktor V Morozov *Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Jan MacháčekInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.
Ekaterina S ShakhovaPlanta LLC, Moscow, Russia.ORCID http://orcid.org/0000-0002-6345-1330
Maxim M PerfilovPlanta LLC, Moscow, Russia.ORCID http://orcid.org/0000-0002-8987-9562
Olga A BelozerovaPlanta LLC, Moscow, Russia.
Sergey I KovalchukShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Kseniia A PalkinaPlanta LLC, Moscow, Russia.
Nikola DrážnáInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.
Zuzana VondrákováInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.
Karel MüllerInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.
Tetiana KalachovaInstitute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.ORCID http://orcid.org/0000-0002-2843-5482
Aubin FleissSynthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK.ORCID http://orcid.org/0000-0003-0126-4537
Josefina Patricia Fernandez-MorenoDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA.
Jose M AlonsoDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA.
Anna N StepanovaDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USA.ORCID http://orcid.org/0000-0003-1018-4758
Liliia I FakhranurovaPlanta LLC, Moscow, Russia.
Nadezhda M MarkinaPlanta LLC, Moscow, Russia.
Dmitry A GorbachevShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Evgenia N BugaevaPlanta LLC, Moscow, Russia.
Galina M DelnovaPlanta LLC, Moscow, Russia.
Vladimir V ChoobPlanta LLC, Moscow, Russia.
Ilia V YampolskyPlanta LLC, Moscow, Russia.ORCID http://orcid.org/0000-0003-2558-2476
Jan Petrášek *Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia. Petrasek@ueb.cas.cz.ORCID http://orcid.org/0000-0002-6719-2735
Alexander S Mishin *Planta LLC, Moscow, Russia. mishin@ibch.ru.ORCID http://orcid.org/0000-0002-4935-7030
Karen S Sarkisyan *Synthetic Biology Group, MRC Laboratory of Medical Sciences, London, UK. karen@light.bio.ORCID http://orcid.org/0000-0002-5375-6341

Funding

Russian Science Foundation (RSF) 24-74-10087Russian Science Foundation (RSF) 25-14-00349Russian Science Foundation (RSF) 25-44-02157Russian Science Foundation (RSF) 25-76-30006
6 · The paper itself

Abstract

Jasmonic and salicylic acids are key hormones involved in plant responses to pests and pathogens. Existing fluorescence-based approaches to imaging plant defence hormones are constrained by the need for external illumination and by autofluorescence of plant tissues, while luminescence-based ones require exogenous substrates. Here, we use jasmonate- and salicylate-responsive promoters to engineer autoluminescent plants that report hormone signalling activity with up to a 53-fold contrast. Using consumer-grade cameras, we image reporter Arabidopsis thaliana and Nicotiana benthamiana plants throughout normal development and in response to pest and pathogen attacks, visualising local and systemic responses. Because the luminescence is self-sustained, these reporters enable non-invasive, substrate-free imaging of defence signalling over extended time courses without specialised equipment.

Indexed as

ArabidopsisNicotianaCyclopentanesGene Expression Regulation, PlantOxylipinsPlant DiseasesPlant Growth RegulatorsPlants, Genetically ModifiedPromoter Regions, GeneticSalicylic AcidSignal TransductionCyclopentanesjasmonic acidOxylipinsPlant Growth RegulatorsSalicylic Acid

Identifiers

PMID41826305
PMCPMC13376918

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.