ArticleAustralian endodontic journal : the journal of the Australian Society of Endodontology Inc2026
Clinical Potential of Long-Term Cryopreserved Stem Cells From Impacted Third Molar Pulps for Regenerative Endodontic Procedures: An Ex Vivo Evaluation.
Article in Australian endodontic journal : the journal of the Australian Society of Endodontology Inc, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study aimed to establish a standardised protocol for isolating dental pulp stem cells (DPSCs) from fully impacted third molar teeth and to assess the effect of long-term cryopreservation on their viability for future clinical applications in regenerative endodontics. DPSCs were isolated from 21 impacted third molars. The clinical relevance of these cells was characterised via flow cytometry, confirming that 96% expressed mesenchymal stem cell markers. Seventeen samples were cryopreserved at -86°C for up to 12 months to evaluate biological stability for autologous transplantation. The standardised surgical extraction and isolation protocol yielded a high concentration of therapeutic-grade cells (3.43 × 10
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