Alteration of Mitochondrial Ca2+ Fluxes by Kaempferol and CGP-37157 Regulates Ca2+ Oscillations in Human Alveolar Type 2 A549 Cells.

K-C Wu, C-Y Chen, L-R Shiao, Z-H Yang, C-M Chuang, Y-M Leung

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Article in Physiological research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

K-C WuDepartment of Physiology, China Medical University, Taichung, Taiwan. ymleung@mail.cmu.edu.tw; Department of Emergency Medicine, China Medical University Hospital, Taichung, Taiwan. cmcsurgeon@yahoo.com.tw.

C-Y Chen

L-R Shiao

Z-H Yang

C-M Chuang

Y-M Leung

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No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mitochondria participate in regulating cytosolic Ca2+ signaling by their Ca2+ handling via mitochondrial Ca2+ uniporter (MCU) and mitochondrial Na+/Ca2+ exchanger (mitoNCX). In this study, we examined how agonist-triggered cytosolic Ca2+ oscillations in human alveolar type 2 A549 cells were affected by an MCU inhibitor (MCU-i4), MCU activator (kaempferol) and mitoNCX inhibitor (CGP-37157). Whilst inhibition of MCU did not significantly repress Ca2+ oscillations, MCU activation by kaempferol considerably dampened oscillatory activities. Inhibition of mitochondrial Ca2+ efflux by CGP-37157 also suppressed Ca2+ oscillations; the suppressive effects of kaempferol and CGP 37157 were not additive. Both kaempferol and CGP-37157 caused a rise in mitochondrial matrix Ca2+ level, but their effects were not additive. Taken together, our results suggest Ca2+ oscillations in alveolar type 2 A549 cells were regulated by stimulating Ca2+ uptake into, and preventing Ca2+ efflux from, the mitochondria, with both cases resulting in disturbed Ca2+ traffic and Ca2+ accumulation in the mitochondrial matrix. Key words Mitochondria " Ca2+ oscillations " Mitochondrial Ca2+ uniporter " Mitochondrial Na+/Ca2+ exchanger " A549 cells.

Indexed as

CalciumCalcium SignalingClonazepamKaempferolsMitochondriaThiazepinesA549 CellsCalcium ChannelsHumansSodium-Calcium ExchangerCalciumCalcium ChannelsCGP 37157ClonazepamkaempferolKaempferolsmitochondrial calcium uniporterSodium-Calcium ExchangerThiazepines

Identifiers

PMID41823096

PMCPMC13128011

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.