ArticleBiology open2026
Customizable FDM-based zebrafish larva mold for live imaging.
Article in Biology open, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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3 authors.
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Abstract
Accurate and reproducible orientation of zebrafish larvae is essential for high-resolution live imaging but remains difficult with standard agarose mounting. Although previous methods have used 3D-printed tools, often fabricated via stereolithography, here, we present an orientation tool made via fused deposition modeling and enhanced with a thin resin coating to improve surface smoothness and performance. This design creates consistent, larval-shaped wells that orient larvae dorsoventrally. The molds, designed to fit standard glass-bottom imaging dishes, are inexpensive to produce on common desktop 3D printers and can be easily customized to fit different imaging setups using the open-source design files. This approach enables reliable, long-term live imaging of multiple larvae and extends the use of low-cost additive manufacturing in zebrafish imaging workflows.
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Registered trials
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