Evidence map›Paper›PMID 41821137›Full record

ArticleBMC pharmacology & toxicology2026

In- vitro assessment of the anti- malarial potential of Alpha Onocerin; cytotoxicity and hemolytic effect, multi-stage activity and molecular docking.

Jemima Aggrey Appiah, Jude Tetteh, Felix Zoiku, Abenaa Owusuwaa Amoatey, Kofi Junior Osei, Sara Agyemang Antwi, John Nii Addotey, Charles Ansah, Kwesi Boadu Mensah

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pharmacology & toxicology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Antiplasmodial Activity of Methylangolensate FromJournal of tropical medicine · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jemima Aggrey AppiahDepartment of Pharmaceutical Science, Kumasi Technical University, Kumasi, Ghana. jemima.otchere@kstu.edu.gh.
Jude TettehDepartment of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Felix ZoikuNoguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
Abenaa Owusuwaa AmoateyDepartment of Pharmaceutical Science, Kumasi Technical University, Kumasi, Ghana.
Kofi Junior OseiDepartment of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Sara Agyemang AntwiDepartment of Pharmaceutical Science, Kumasi Technical University, Kumasi, Ghana.
John Nii AddoteyDepartment of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Charles AnsahDepartment of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Kwesi Boadu MensahDepartment of Pharmacology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The surfacing of resistant strains of Plasmodium falciparum (P. falciparum) against various antimalarials on the market has intensified the need for novel antimalarial agents, particularly those with multistage efficacy. This study investigated the safety and anti-plasmodial efficacy of alpha onocerin (AOC), a triterpenoid compound derived from Huperzia phlegmaria, against both 3D7 and Dd2 lab strains of P. falciparum. In vitro anti-plasmodial assays were carried out to investigate the activity of AOC across trophozoite, schizont, and gametocyte stages. The SYBR Green assay was used to quantify parasite growth after 72 h of incubation. The cytotoxic effect of AOC was tested against the HepG2 cell using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. AOC exhibited significant stage-specific anti-plasmodial activity, with IC₅₀ values of 2.30 ± 0.44 µM (trophozoites) and 3.96 ± 0.72 µM (schizonts) for 3D7, with Dd2 yielding 6.33 ± 0.63 µM (trophozoites) and 13.76 ± 1.09 (schizonts). Molecular docking further revealed potential interactions of AOC with parasite targets, suggesting a probable mechanism involving disruption of parasite metabolism or membrane integrity. These findings underscore the promise of the compound as a viable lead candidate for antimalarial drug development, supported by its broad inhibitory activity against P. falciparum.

Indexed as

AntimalarialsPlasmodium falciparumTriterpenesCell SurvivalErythrocytesHemolysisHep G2 CellsHumansMolecular Docking SimulationAntimalarialsTriterpenesAlpha onocerinAnti-plasmodial activityDrug resistanceHemolysisMolecular dockingMultistage malariaNatural productPlasmodium falciparumTriterpenoids

Identifiers

PMID41821137
PMCPMC13097737

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.