ArticleNature methods2026
Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.
Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Tartrazine Clears Live Cells while Preserving Viability at High Refractive Indices and Osmolality.Bioconjugate chemistry · 2026Article
- Tartrazine clears live cells while preserving viability at high refractive indices and osmolality.bioRxiv : the preprint server for biology · 2026Article
- Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.Nature methods · 2026Article
- Dendritic compartment-specific spine formation in layer 5 neurons underlies cortical circuit maturation during adolescence.Science advances · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- Advances in organoid imaging and automated morphometric analysis: from optical microscopy to computational approaches.Frontiers in cell and developmental biology · 2026Review
- A simple and versatile plasma membrane staining method for visualizing living cell morphology in reproductive tissues across diverse plant species.Plant methods · 2025Article
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Authors and funding
21 authors.
Funding
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Abstract
Tissue clearing has been widely used for fluorescence imaging of fixed tissues, but its application to live tissues has been limited by toxicity. Here we develop minimally invasive optical clearing media for fluorescence imaging of live mammalian tissues. Light scattering is minimized by adding spherical polymers with low osmolarity to the extracellular medium. A clearing medium containing bovine serum albumin (SeeDB-Live) is compatible with live cells, enabling structural and functional imaging of live tissues, such as spheroids, organoids, acute brain slices and the mouse brains in vivo. SeeDB-Live minimally affects neuronal electrophysiological properties and sensory responses in vivo, and facilitates fluorescence imaging of deep cortical layers in live animals without detectable toxicity to neurons or behavior. We further demonstrate its utility to epifluorescence voltage imaging in acute brain slices and in vivo preparations. Thus, SeeDB-Live expands both the depth and modality range of fluorescence imaging in live mammalian tissues.
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Registered trials
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