ArticleNature communications2026
Discovery and engineering of bacterial P450s for C-14 hydroxylation in ent-kaurane diterpenoids.
Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Rational Engineering of AKR13B3 fromInternational journal of molecular sciences · 2026Article
- A Review of Plant-Derived Diterpenoid Biosynthesis: From Structural Scaffold Diversity and Lineage-Associated Distribution to Enzyme Mining and Discovery Strategies.Molecules (Basel, Switzerland) · 2026Review
- Reconstruction ofEngineering in life sciences · 2026Article
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Authors and funding
13 authors.
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Abstract
ent-Kaurane diterpenoids (ent-KTs) represent a structurally diverse class of natural products renowned for their antitumor and anti-inflammatory bioactivities. The C-14 hydroxyl modification is crucial for enhancing their potency; however, introducing this functional group remains a considerable challenge. Here, we present a computational heme-guided site-specific (CHS) strategy to identify three bacterial P450s (CYP260A1, CYP105N1, and CYP154C5) for C-14 hydroxylation. Further computationally guided enzyme engineering and redox partner screening identify the CYP260A1 L162V variant paired with CamA/CamB, achieving a 52-fold increase in production titer and a yield of 84.2 mg/L of (14R,16R)-ent-kauran-14,16-diol (2) in Escherichia coli. Substrate scope test reveals functional groups affecting reactivity. Structure-activity relationship studies demonstrate the synergistic effect between the C-14 hydroxyl and C15-C16 Michael acceptor, resulting in a potent derivative (27) with strong cytotoxicity (IC
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