ArticleSTAR protocols2026
Protocol for precision cutting and short-term culture of lymph node tissue slices for modeling of innate immune responses ex vivo.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Secondary lymphoid organs are highly organized for the initiation of immune responses. Here, we present a protocol that enables this microanatomical structure to be preserved during short-term culture, allowing ex vivo modeling of human innate immune responses. We describe the steps for precision cutting of human lymph nodes into live tissue cross-sections that can be cultured for up to 20 h with immune stimuli and analyzed by multiple readouts. For complete details on the use and execution of this protocol, please refer to Fergusson et al.
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