ArticleSTAR protocols2026
Protocol for stable cell line production to express muscle-type nicotinic receptor.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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3 authors.
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Abstract
The adult muscle-type nicotinic acetylcholine receptor (AChR) is essential for neuromuscular transmission but is difficult to produce due to the requirement for coordinated subunit assembly. Here, we present a protocol for generating doxycycline-inducible stable cell lines that co-express all four subunits. We describe steps for lentivirus infection, puromycin selection, fluorescence-activated cell sorting, clonal expansion, and protein expression test. This protocol enables AChR production at quantities sufficient for single-particle cryoelectron microscopy (cryo-EM) and may be applicable to other hetero-multimeric protein complexes. For complete details on the use and execution of this protocol, please refer to Li et al.
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