Evidence map›Paper›PMID 41818235›Full record

ArticleSTAR protocols2026

Protocol to analyze steps of ribosome biogenesis in nuclear and cytosolic fractions of yeast cells.

Ramin Zadali, Ulrike Topf

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ramin ZadaliInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland; Doctoral School of Molecular Biology and Biological Chemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland. Electronic address: rzadalimomen@ibb.waw.pl.
Ulrike TopfInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland. Electronic address: utopf@ibb.waw.pl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell fractionation enables the study of compartment-specific RNA processing events in organisms with complex cell walls. Here, we present a protocol for isolating nuclear and cytosolic fractions from Saccharomyces cerevisiae. We describe spheroplast formation using zymolyase, followed by subcellular fractionation via a sucrose density gradient. Finally, we detail the extraction of high-quality RNA from these fractions. This approach overcomes cell wall barriers to allow the analysis of ribosome biogenesis. For complete details on the use and execution of this protocol, please refer to Zadali et al.

Indexed as

Cell FractionationCell NucleusCytosolRibosomesSaccharomyces cerevisiaeCell biologyCell separation/fractionationMolecular biologyProtein biochemistry

Identifiers

PMID41818235
PMCPMC12997206

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.