Evidence map›Paper›PMID 41817354›Full record

ArticlePlant biotechnology journal2026

Cross-Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana-Derived WUS2 Developmental Regulator.

Roni Chaudhary, Surender Singh, Usman Ali, Siddharth Tiwari

Abstract read
In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Roni ChaudharyPlant Tissue Culture and Genetic Engineering Lab, BRIC-National Agri-Food and Biomanufacturing Institute (BRIC-NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), Mohali, Punjab, India.
Surender SinghPlant Tissue Culture and Genetic Engineering Lab, BRIC-National Agri-Food and Biomanufacturing Institute (BRIC-NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), Mohali, Punjab, India.
Usman AliPlant Tissue Culture and Genetic Engineering Lab, BRIC-National Agri-Food and Biomanufacturing Institute (BRIC-NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), Mohali, Punjab, India.
Siddharth TiwariPlant Tissue Culture and Genetic Engineering Lab, BRIC-National Agri-Food and Biomanufacturing Institute (BRIC-NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India), Mohali, Punjab, India.ORCID https://orcid.org/0000-0003-3958-8465

Funding

Biotechnology Industry Research Assistance Council (BIRAC)BRIC-National Agri-Food and Biomanufacturing Institute (BRIC-NABI)Department of Biotechnology (DBT), Ministry of Science and Technology, India BT/PR25789/GET/119/97/2017Department of Biotechnology (DBT), Ministry of Science and Technology, India BT/PR51096/NER/95/1987/2023
6 · The paper itself

Abstract

Plant regeneration is governed by intrinsic gene regulation and phytohormonal cues. WUSCHEL (WUS) gene promotes regeneration, but its broader functional role remains unexplored. Here, we demonstrate that the constitutive and inducible expression of banana-derived WUS2 (GN-WUS2) enhances regeneration in Nicotiana tabacum (tobacco) and Musa acuminata (banana) cv. Grand Naine, even in hormone-free MS medium. Constitutive (CaMV35S::GN-WUS2) expression promoted shoot formation and modulated hormonal and morphogenic gene expression, as evidenced by molecular, biochemical and histological analyses. However, it caused some pleiotropic effects. To overcome this, glucocorticoid receptor-based inducible GN-WUS2 expression enabled healthy shoot development. The upregulated expression of HMGR1, IPPI2 and SMT1-2 in transgenic tobacco lines boosted isoprenoid and phytosterol biosynthesis and correlated with increased cell division, biomass, pod size and seed yield. Proteomic analysis of seeds from transgenic tobacco lines revealed an enrichment of lipid-associated proteins and the accumulation of the novel lipid adipic acid, supported by expression profiling of NtKA and NtSA genes. Collectively, these results establish GN-WUS2 as a master regulator that integrates developmental reprogramming with novel phytosterol biosynthesis and yield enhancement, presenting its versatile role in next-generation regeneration and crop improvement.

Indexed as

Homeodomain ProteinsMusaNicotianaPlant ProteinsGene Expression Regulation, PlantPlants, Genetically ModifiedPlant ShootsHomeodomain ProteinsPlant Proteinsbananadevelopmental regulatorsectopic expressiongenetic transformationinducible expressionlipidsphytohormonestobaccoWUS

Identifiers

PMID41817354
PMCPMC13205890

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.