ArticleFrontiers in bioengineering and biotechnology2026
Impact of keto leucine and isoleucine on CHO cell central carbon metabolism and performance in fed-batch and steady-state perfusion.
Article in Frontiers in bioengineering and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The keto acids of isoleucine and leucine are bioavailable precursors of their branched-chain amino acids in Chinese hamster ovary (CHO) cells, which are used to produce biotherapeutics at industrial scale. In this study, the potential of branched-chain keto acids to improve product yield was evaluated in fed-batch and simulated steady-state perfusion. In fed-batch, combined or individual replacement of isoleucine and leucine at equimolar concentrations with their keto acids moderately increased (+6%) or maintained the cell-specific productivity
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