Evidence map›Paper›PMID 41814427›Full record

ArticleGenome biology2026

Proteomics and tracer metabolomics link GAPDH ISGylation to glycolytic control.

Denzel Eggermont, Lissa Eggermont, Nagihan Aslantaş, Fabien Thery, Katie Boucher, Antje Beling, Bart Ghesquière, Francis Impens

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In one paragraph

Article in Genome biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Denzel EggermontVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
Lissa EggermontVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
Nagihan AslantaşDepartment of Biomolecular Medicine, Ghent University, Ghent, Belgium.
Fabien TheryVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
Katie BoucherVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium.
Antje BelingCharité, Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Institute of Biochemistry, Berlin, Germany.
Bart GhesquièreMetabolomics Core Facility Leuven, VIB Center for Cancer Biology, VIB, Louvain, Belgium.
Francis ImpensVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium. francis.impens@vib-ugent.be.

Funding

ERANET Infect-ERA BACVIRISG15European Research Council 101089193Fonds Wetenschappelijk Onderzoek 1167223NFonds Wetenschappelijk Onderzoek 12AN524NFonds Wetenschappelijk Onderzoek G0F8616N
6 · The paper itself

Abstract

backgroundUbiquitin-like protein ISG15 (interferon-stimulated gene 15) is implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems limit mechanistic insight. Here, we apply a multi-omics approach in cells ectopically expressing the ISGylation machinery independent of immune stimuli, to generate a systematic view of ISGylation in metabolic control.

resultsISGylation preferentially targets metabolic enzymes, with marked enrichment among glycolytic proteins, suppressing the energy-yielding phase of glycolysis. Tracer metabolomics reveals a bottleneck at glyceraldehyde-3-phosphate dehydrogenase (GAPDH), reflected by accumulation of upstream intermediates and depletion of downstream metabolites. This arises from multisite ISGylation of lysines near its catalytic and regulatory regions, which reduces enzymatic activity without disrupting tetramer assembly.

conclusionsThese findings identify GAPDH as a central metabolic checkpoint regulated by ISGylation and uncover a direct post-translational mechanism by which ISG15 controls energy metabolism.

Indexed as

CytokinesGlyceraldehyde-3-Phosphate DehydrogenasesGlycolysisMetabolomicsProteomicsUbiquitinsAnimalsHumansProtein Processing, Post-TranslationalCytokinesGlyceraldehyde-3-Phosphate DehydrogenasesISG15 protein, humanUbiquitinsGAPDHGlycolysisISG15Mass spectrometryMetabolomicsProteomics

Identifiers

PMID41814427
PMCPMC13093933

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.