Evidence map›Paper›PMID 41814080›Full record

ArticleMycopathologia2026

Molecular detection of medically relevant Sporothrix species in roadkilled wildlife in the Brazilian Atlantic forest.

Steffanie Skau Amadei, Julia Campos, Andressa Maria Rorato Nascimento de Matos, Keity Aparecida Speçato, Eloiza Teles Caldart, Ana Paula Frederico Rodrigues Loureiro Bracarense, Ferry Hagen, Zoilo Pires de Camargo, Anderson Messias Rodrigues

Abstract read
In one paragraph

Article in Mycopathologia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Steffanie Skau AmadeiIntegrated Program in Neuroscience, McGill University, Montreal, Canada.ORCID http://orcid.org/0000-0001-5503-1566
Julia CamposSchool of Medicine and Life Sciences, Pontifical Catholic University of Paraná, Curitiba, Brazil.ORCID http://orcid.org/0009-0001-5412-0425
Andressa Maria Rorato Nascimento de MatosDepartment of Preventive Veterinary Medicine, State University of Londrina, Londrina, Brazil.ORCID http://orcid.org/0000-0002-4095-2167
Keity Aparecida SpeçatoDepartment of Preventive Veterinary Medicine, State University of Londrina, Londrina, Brazil.ORCID http://orcid.org/0009-0004-2651-439X
Eloiza Teles CaldartDepartment of Preventive Veterinary Medicine, State University of Londrina, Londrina, Brazil.ORCID http://orcid.org/0000-0002-0475-2010
Ana Paula Frederico Rodrigues Loureiro BracarenseDepartment of Preventive Veterinary Medicine, State University of Londrina, Londrina, Brazil.ORCID http://orcid.org/0000-0002-5407-2582
Ferry HagenDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.ORCID http://orcid.org/0000-0002-5622-1916
Zoilo Pires de CamargoLaboratory of Emerging Fungal Pathogens, Department of Microbiology, Immunology, and Parasitology, Discipline of Cellular Biology, Federal University of São Paulo (UNIFESP), São Paulo, 04023062, Brazil.ORCID http://orcid.org/0000-0002-2971-7983
Anderson Messias RodriguesLaboratory of Emerging Fungal Pathogens, Department of Microbiology, Immunology, and Parasitology, Discipline of Cellular Biology, Federal University of São Paulo (UNIFESP), São Paulo, 04023062, Brazil. amrodrigues@unifesp.br.ORCID http://orcid.org/0000-0003-0891-4563

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 406639/2025-6Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887.159096/2017-00Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/27265-5
6 · The paper itself

Abstract

The rapid expansion of zoonotic sporotrichosis in South America necessitates innovative surveillance strategies to identify natural ecological niches. Roadkill provides a unique, underutilized opportunity to monitor Sporothrix circulation within human-impacted landscapes. We conducted a molecular survey via a triplex probe quantitative real-time PCR (qPCR) assay targeting pathogenic Sporothrix species in 81 roadkilled vertebrates (mammals, birds, and reptiles) collected along highways BR-376 and PR-445 traversing the Atlantic Forest in Paraná, Brazil (2017-2023). Genomic DNA from visceral organs (heart, liver, lung, and spleen) was screened for fungal DNA. Sporothrix DNA was detected in 13.6% (11/81) of the samples. Sporothrix schenckii predominated, identified in wild mammals (Leopardus guttulus, Didelphis albiventris, and Lepus europaeus) and diverse birds (Colaptes melanochloros, Piaya cayana, and Selenidera maculirostris), indicating systemic exposure. Strikingly, S. brasiliensis was detected in avian hosts (Columbina picui, Crypturellus tataupa), challenging the thermal-exclusion hypothesis and implicating birds as potential aerial vectors. Furthermore, S. globosa was found in Dasyprocta spp. and was co-detected with S. brasiliensis in Columbina picui. Notably, a reptile (Oxyrhopus spp.) was positive for S. globosa and S. schenckii, expanding the known host spectrum, potentially via trophic transmission. Although distinguishing transient DNA carriage from active infection requires histopathological validation, these findings suggest that wildlife in fragmented corridors may function as reservoirs, biological amplifiers, or mechanical vectors rather than incidental hosts, reinforcing the blurring boundaries between sylvatic and synanthropic transmission cycles. In this context, integrating roadkill biosurveillance into a One Health framework is vital for tracking environmental pathogen loads and anticipating zoonotic spillover.

Indexed as

Animals, WildSporothrixSporotrichosisAnimalsBirdsBrazilDNA, FungalForestsHumansMammalsReal-Time Polymerase Chain ReactionDNA, FungalRoadkillSporothrix brasiliensisSporothrix globosaSporothrix schenckiiSporotrichosisWildlife

Identifiers

PMID41814080
PMCPMC12979292

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.