Evidence map›Paper›PMID 41813865›Full record

ArticleApplied microbiology and biotechnology2026

Natural food-derived antimicrobials against beer spoilage microorganisms.

Chang Liu, Jiale Tang, Peng Lu, Satoka Takabayashi, Dai Yagaki, Kenta Tsutsumi, Suguru Okuda, Hideaki Itoh, Hidenori Matsukura, Saki Toyota and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Chang LiuDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Jiale TangDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Peng LuDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Satoka TakabayashiDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Dai YagakiDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Kenta TsutsumiDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Suguru OkudaDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Hideaki ItohDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Hidenori MatsukuraResearch Laboratories for Alcohol Beverages, Asahi Breweries Ltd, 1-21 Midori, 1-Chome, Moriya-Shi, Ibaraki, 302-0106, Japan.
Saki ToyotaResearch Laboratories for Alcohol Beverages, Asahi Breweries Ltd, 1-21 Midori, 1-Chome, Moriya-Shi, Ibaraki, 302-0106, Japan.
Masaki ShimokawaResearch Laboratories for Alcohol Beverages, Asahi Breweries Ltd, 1-21 Midori, 1-Chome, Moriya-Shi, Ibaraki, 302-0106, Japan.
Koji SuzukiOpen Innovation Team, Asahi Quality & Innovations, Ltd, 1-21 Midori, 1-Chome, Moriya-Shi, Ibaraki, 302-0106, Japan.
Koji NagataDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan. aknagata@mail.ecc.u-tokyo.ac.jp.

Funding

Asahi Quality & Innovations, Ltd. and Asahi Breweries, Ltd. GSALS-R3-39
6 · The paper itself

Abstract

The spoilage of beer significantly threatens the quality and safety of products in the beverage industry. Certain natural foods contain beneficial bioactive components that are considered to be safer than chemical additives. This study aimed to identify extracts of natural foods as potential alternatives to chemical preservatives for controlling beer spoilage microorganisms. Among the two extraction methods applied to 176 natural foods, the extracts of clove alone effectively inhibited the growth of representative beer spoilage microorganisms, specifically Levilactobacillus brevis, Sporolactobacillus vineae, Pectinatus frisingensis, and Saccharomyces cerevisiae var. diastaticus. High-performance liquid chromatography and half-maximal inhibitory concentration analysis revealed that gallic acid and eugenol in cloves were active compounds with antimicrobial properties in vitro that were of a similar order of magnitude to those of commercial preservatives (potassium sorbate and sodium benzoate) under the tested conditions. Scanning electron microscopy observations and a fluorescence leakage assay using 3,3'-dipropylthiadicarbocyanine iodide indicated morphological alterations and membrane perturbation in treated microorganisms, except for the limited effect of gallic acid on S. cerevisiae var. diastaticus. These findings provide insight into the potential role of natural food-derived antimicrobials in controlling beer spoilage microorganisms, pending further validation in real beverage systems.

Indexed as

Anti-Infective AgentsBeerFood PreservativesPlant ExtractsSyzygiumChromatography, High Pressure LiquidEugenolFood MicrobiologyGallic AcidMicrobial Sensitivity TestsSaccharomyces cerevisiaeAnti-Infective AgentsEugenolFood PreservativesGallic AcidPlant ExtractsBeer spoilage microorganismsCloveEugenolGallic acidMembrane perturbationNatural preservatives

Identifiers

PMID41813865
PMCPMC12982314

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.