ArticleScientific reports2026
An advanced rapid-visual CRISPR assay for detecting porcine reproductive and respiratory syndrome virus.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The porcine reproductive and respiratory syndrome virus (PRRSV) remains a significant threat to the global swine industry, underscoring the urgent need for innovative diagnostic methods to detect and manage outbreaks effectively. We developed a novel CRISPR-based fluorescence assay for the highly sensitive detection of PRRSV-2. By combining reverse transcription-recombinase polymerase amplification (RT-RPA) with multiple-crRNA CRISPR/Cas13a system and single-stranded RNA-fluorescently quenched reporters (RQ-5U), our assay achieved a significant 28-fold increase in sensitivity compared to existed CRISPR/Cas13a-based PRRSV-2 detection methods. This multiple crRNA strategy allows detecting as low as 6 copies/µL of PRRSV-2 RNA, significantly improving the detection limit. Moreover, our method's accuracy in detecting simulated PRRSV-2 clinical samples matches that of quantitative reverse transcription polymerase chain reaction (RT-qPCR). Our findings demonstrate that this visual, sensitive, and specific nucleic acid detection method holds great promise for enhancing the diagnosis and management of PRRS in the swine industry.
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