ArticleCell reports. Medicine2026
A targeted proteomics assay for the preoperative diagnosis of thyroid nodules.
Abstract readMulticenter Study
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Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2citing papers in PubMed
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2 citing papers in PubMed.
- Article
- Association of age, BMI and their interaction with thyroid nodules in patients with type 2 diabetes mellitus: a cross-sectional study.Frontiers in endocrinology · 2026Article
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Authors and funding
50 authors.
Xue CaiAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, China.
Yaoting SunAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, China.
Dingcun LuoDepartment of Oncological Surgical, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou, Zhejiang, China.
Hao ZhangDepartment of Thyroid Surgery, the First Hospital of China Medical University, Shenyang, Liaoning, China.
Wenjun WeiDepartment of Head and Neck Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
Yifeng ZhangDepartment of Medical Ultrasound, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Weigang GeWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Jing LiuFirst Hospital of Shanxi Medical University, Thyroid Surgery Department, Taiyuan, Shanxi, China.
Guan RuanWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Lu LiAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, China.
Lingling TanWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Luang XuWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Dong XuDepartment of Ultrasound, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, China.
Haixia GuanDepartment of Endocrinology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.
Jiang ZhuWomen's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Wanyuan ChenDepartment of Pathology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
Qiushi ZhangWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Huijuan SiWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Juntao QiuWestlake Omics (Hangzhou) Biotechnology Co., Ltd., No.1, Yunmeng Road, Cloud Town, Xihu District, Hangzhou, Zhejiang, China.
Zhangzhi XueAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, China.
Zhiqiang GuiDepartment of Thyroid Surgery, the First Hospital of China Medical University, Shenyang, Liaoning, China.
Haitao ZhengDepartment of Thyroid Surgery, Affiliated Yantai Yuhuangding Hospital, Qingdao University, Shandong, China.
Gaosong WuDepartment of Thyroid and Breast Surgery, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan, Hubei, China.
Jianhua WangDepartment of Thyroid and Breast Surgery, Affiliated Hospital of Integrated Chinese and Western, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
Lei XieDepartment of Head and Neck surgery, Institute of Micro-Invasive Surgery of Zhejiang University, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Guang ChenDepartment of Thyroid Surgery, General Surgery Center, The First Hospital of Jilin University, Changchun, Shenyang, China.
Guoyang WuDepartment of General Surgery, Zhongshan Hospital Affiliated with Xiamen University, Xiamen, China.
Lei LiangDepartment of Ultrasound, Aerospace Center Hospital, Beijing, China.
Chuang ChenDepartment of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
Xianghui HeDepartment of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
Meiping ShenDepartment of General Surgery, the First Affiliated of Nanjing Medical University, Nanjing, Jiangsu, China.
Jianbiao WangDepartment of Head and Neck surgery, Institute of Micro-Invasive Surgery of Zhejiang University, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Minghua GeDepartment of Pathology, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
Deguang ZhangDepartment of Head and Neck surgery, Institute of Micro-Invasive Surgery of Zhejiang University, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Liang ZhouDepartment of Head and Neck surgery, Institute of Micro-Invasive Surgery of Zhejiang University, The Affiliated Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Yijun WuDepartment of Thyroid Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Hui ShiDepartment of Medical Ultrasound, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Fan WuDepartment of Oncological Surgical, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou, Zhejiang, China.
Zhihong WangDepartment of Thyroid Surgery, the First Hospital of China Medical University, Shenyang, Liaoning, China.
Hong HanDepartment of Ultrasound, Zhongshan Hospital, Institute of Ultrasound in Medicine and Engineering, Fudan University, Shanghai, China.
Jing YaoDepartment of Thyroid & Hernia Surgery, Medical Department of General Surgery, The First Medical Center of Chinese People's Liberation Army General Hospital, Beijing, China.
Zhiyan LiuDepartment of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Mingzhao XingSchool of Medicine, Southern University of Science and Technology, Shenzhen, Guangdong, China.
Kennichi KakudoDepartment of Pathology, Cancer Genome Center and Thyroid Disease Center, Izumi City General Hospital, Izumi, Japan.
Yibo GaoCentral Laboratory & Shenzhen Key Laboratory of Epigenetics and Precision Medicine for Cancers, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen 518116, China.
Wen TianDepartment of Thyroid & Hernia Surgery, Medical Department of General Surgery, The First Medical Center of Chinese People's Liberation Army General Hospital, Beijing, China. Electronic address: tianwen301_cta01@163.com.
Huixiong XuDepartment of Ultrasound, Zhongshan Hospital, Institute of Ultrasound in Medicine and Engineering, Fudan University, Shanghai, China. Electronic address: xuhuixiong@126.com.
Yi ZhuAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, China. Electronic address: zhuyi@westlake.edu.cn.
Yu WangDepartment of Head and Neck Surgery, Fudan University Shanghai Cancer Center; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China. Electronic address: neck130@sina.com.
Tiannan GuoAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; Research Center for Industries of the Future, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang, China. Electronic address: guotiannan@westlake.edu.cn.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate preoperative diagnosis of thyroid nodules via fine-needle aspiration (FNA) biopsy remains challenging, particularly in cases with indeterminate cytology. This prospective, noninterventional, blinded, multicenter study establishes ThyroProt, a diagnostic classifier that integrates targeted mass-spectrometry-based quantification of a 3-protein signature with BRAF V600E mutation status, age, and gender. Developed and validated on 837 FNA samples, the classifier is evaluated in a prospective test set of 322 samples, achieving an area under the curve (AUC) of 0.94 with an overall accuracy of 90.7%. For the critical subgroup of Bethesda III/IV nodules, ThyroProt demonstrates an accuracy of 88.0%, with 82.4% sensitivity and 100% specificity. The classifier's robust performance is further evaluated in two independent multicenter cohorts, where it maintains an AUC of 0.87-0.91 and an accuracy of 84.3%-85.7%. This study supports the clinical utility of mass-spectrometry-based targeted proteomics for improving preoperative diagnosis of thyroid nodules, particularly those with indeterminate cytology.
Indexed as
ProteomicsThyroid NoduleAdultAgedBiopsy, Fine-NeedleFemaleHumansMaleMass SpectrometryMiddle AgedMutationProspective StudiesProto-Oncogene Proteins B-rafProto-Oncogene Proteins B-rafabsolute quantificationartificial intelligencebethesda classificationfine-needle aspirationindeterminate thyroid nodulesmass spectrometrymolecular classifier modelmulti-center clinical studyprospective diagnosistargeted proteomics
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.