Evidence map›Paper›PMID 41811904›Full record

ArticleJournal of immunology research2026

OPN-Derived Peptides Generated by Proteasomes Can Promote Cell Migration via CD44 Activation.

Hindh Imad, Kathryn E Strange, Chiara Dianzani, Wai Tuck Soh, Sophie Siddons, Shahram Kordasti, Casimiro Luca Gigliotti, Annalisa Chiocchetti, Henning Urlaub, Timothy James Nott and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunology research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Hindh ImadResearch Group of Molecular Immunology, The Francis Crick Institute, London, NW1 1AT, UK, crick.ac.uk.
Kathryn E StrangeResearch Group of Molecular Immunology, The Francis Crick Institute, London, NW1 1AT, UK, crick.ac.uk.
Chiara DianzaniDepartment of Drug Science and Technology, University of Turin, 10126, Torino, Italy, unito.it.
Wai Tuck SohResearch Group of Quantitative System Biology, Max-Planck-Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany, uni-goettingen.de.
Sophie SiddonsResearch Group of Molecular Immunology, The Francis Crick Institute, London, NW1 1AT, UK, crick.ac.uk.
Shahram KordastiSchool of Cancer and Pharmaceutical Sciences, King's College, London, UK, kcl.ac.uk.
Casimiro Luca GigliottiInterdisciplinary Research Centre of Autoimmune Diseases (IRCAD), University of Piemonte Orientale, Amedeo Avogadro, 28100, Novara, Italy, uniupo.it.
Annalisa ChiocchettiDepartment Health Sciences, Università Piemonte Orientale, Novara, Italy, uniupo.it.
Henning UrlaubResearch Group of Bioanalytical Mass Spectrometry, Max-Planck-Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany, uni-goettingen.de.
Timothy James NottDepartment of Chemistry, King's College London, London, SE1 1DB, UK, kcl.ac.uk.
Umberto DianzaniInterdisciplinary Research Centre of Autoimmune Diseases (IRCAD), University of Piemonte Orientale, Amedeo Avogadro, 28100, Novara, Italy, uniupo.it.
Juliane LiepeResearch Group of Quantitative System Biology, Max-Planck-Institute for Multidisciplinary Sciences, 37077, Göttingen, Germany, uni-goettingen.de.ORCID https://orcid.org/0000-0003-2515-9707
Michele MishtoResearch Group of Molecular Immunology, The Francis Crick Institute, London, NW1 1AT, UK, crick.ac.uk.ORCID https://orcid.org/0000-0003-3042-2792

Funding

Blood Cancer UK 22009Cancer Research UK CC0102CRUK City of London Centre (CoL) CTRQQR-2021/100004ERC-StG 945528 IMAPEuropean Union's Framework Programme for Research and Innovation Horizon Europe 2021-2027H2020 Marie Skłodowska-Curie Actions 101065466Max Planck SocietyMedical Research CouncilWellcome Trust CC0102
6 · The paper itself

Abstract

Osteopontin (OPN) is a pleiotropic cytokine that is overexpressed in many autoimmune diseases and solid cancers. Here we show that the 20S proteasome can degrade, in vitro, both the full-length OPN and the OPN-C fragment generated in the extracellular space and produce both canonical and spliced peptides. Specific canonical OPN-derived peptides, generated from hotspots within the OPN sequences, can regulate cell migration via CD44. By predicting peptide-protein docking, we propose that this could occur by displacing the flexible C-terminal region from the CD44 α1 helix, promoting a conformational change in CD44 that could lead to the activation of downstream signalling pathways. We also identify key amino acid residues within these OPN-derived peptides that impact the activation of CD44 to initiate cell migration and predict their binding to the CD44 asparagine 164 residue-previously implicated in stabilising the CD44 structure to promote cell migration. Therefore, we propose that proteasomes can process various OPN isoforms, theoretically both inside and outside a cell, leading to the presence of OPN-derived peptides in the extracellular space where they can play an immunological role. This process, which others showed to represent an antibacterial defence, could also be involved in the regulation of cell surface receptors.

Indexed as

Hyaluronan ReceptorsOsteopontinPeptidesProteasome Endopeptidase ComplexAmino Acid SequenceAnimalsCell MovementHumansProtein BindingProtein IsoformsProteolysisSignal TransductionCD44 protein, humanHyaluronan ReceptorsOsteopontinPeptidesProteasome Endopeptidase ComplexProtein Isoforms20S proteasomeCD44disordered proteinsinSPIRElymphocytesmigrationosteopontinpeptide splicing

Identifiers

PMID41811904
PMCPMC13140175

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.