Evidence map›Paper›PMID 41811845›Full record

ArticleSTAR protocols2026

Protocol to annotate dendritic cell maturation types in vivo making use of lipid nanoparticle-based approaches.

Karo Van Lil, Janne Swinnen, Greta R Webb, Sofie Rennen, Victor Bosteels, Sandra Maréchal, Clint De Nolf, Jessica Vetters, Jiří Březina, Evelien Van De Velde and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Karo Van LilLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Janne SwinnenDepartment of Pharmaceutics, Ghent University, Ghent, Belgium.
Greta R WebbLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Sofie RennenLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Victor BosteelsLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Sandra MaréchalLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Clint De NolfLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium; Laboratory for Barriers in Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
Jessica VettersLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Jiří BřezinaLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Evelien Van De VeldeLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Farzaneh FayazpourLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium.
Rein VerbekeDepartment of Pharmaceutics, Ghent University, Ghent, Belgium.
Ine LentackerDepartment of Pharmaceutics, Ghent University, Ghent, Belgium.
Sophie JanssensLaboratory for ER Stress and Inflammation, VIB Center for Inflammation Research, Ghent, Belgium; Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium. Electronic address: sophie.janssens@irc.vib-ugent.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The current panels used to annotate mature dendritic cells (DCs) by flow cytometry do not suffice to deconvolute homeostatic from immunogenic mature DCs. Here, we present a protocol to classify DCs along a homeostatic/immunogenic spectrum making use of flow cytometry and/or transcriptional profiling. We describe steps to steer DC maturation in vivo using a lipid nanoparticle (LNP)-based approach. We detail procedures to formulate, characterize, and generate these LNPs and outline their application in mice. For complete details on the use and execution of this protocol, please refer to Rennen et al.

Indexed as

Dendritic CellsFlow CytometryLipidsNanoparticlesAnimalsCell DifferentiationLiposomesMiceLipid NanoparticlesLipidsLiposomesCell BiologyCell isolationFlow CytometryGene ExpressionImmunologyProtein Biochemistry

Identifiers

PMID41811845
PMCPMC12994025

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.