Evidence map›Paper›PMID 41811204›Full record

ArticleVeterinary medicine and science2026

Impact of Soybean Meal, Mustard Meal, Rapeseed Meal and Black Cumin on Production Performance, Egg Quality and Gut Microflora of Laying Hens.

Md Abubakar Siddik, Mst Afroza Khatun, Sweety Rani Mondal, Shoriful Islam, Md Azizul Haque, Khadiza Akter Brishty, Hemayet Hossain, Md Mahfujur Rahman

Abstract read
In one paragraph

Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Md Abubakar SiddikDepartment of Dairy and Poultry Science, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Mst Afroza KhatunDepartment of Dairy and Poultry Science, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Sweety Rani MondalDepartment of Dairy and Poultry Science, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Shoriful IslamDepartment of Dairy and Poultry Science, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Md Azizul HaqueDepartment of Dairy and Poultry Science, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Khadiza Akter BrishtyDepartment of Zoology (GSSC), University of Dhaka, Dhaka, Bangladesh.
Hemayet HossainDepartment of Veterinary Science and Animal Husbandry, Teesta University, Rangpur, Bangladesh.ORCID https://orcid.org/0009-0007-4698-3128
Md Mahfujur RahmanDepartment of Medicine; Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID https://orcid.org/0000-0003-2062-8471

Funding

Hajee Mohammad Danesh Science and Technology University
6 · The paper itself

Abstract

BACKGROUND AND

objectivesThis study aimed to assess the impact of diverse protein sources (soybean, mustard, rapeseed and black cumin [BC]) as dietary supplements on production performance, egg quality and gut microflora of Shaver Brown 579 commercial layers.

methodsA randomized design allocated 324 hens over 4 months into six treatments with six replications for each treatment, each replication containing 09 birds. The treatments included T0 (control diet), T1 (basal diet + soybean), T2 (basal diet + mustard), T3 (basal diet + rapeseed), T4 (basal diet + mustard + BC) and T5 (Basal diet + Rapeseed + Black cumin). Production performances were assessed monthly and egg quality characters were assessed at 44th and 52nd weeks.

resultsHens on diets featuring mustard, rapeseed and BC exhibited significantly higher body weight gain (BWG), without substantial impact on feed intake. The highest final body weight was observed in T5, with no mortality occurrences. No significant differences emerged in egg production, albumen index, shape index, shell breaking strength, shell thickness or shell percentage compared to control groups. However, egg weight showed significance in the third month (p < 0.05). Notably, dietary protein sources influenced gut microflora significantly (p < 0.05), with T0 having the highest microbial load and T5 the lowest. Egg production cost was the lowest in T4 (7.13 Tk. or $0.067/egg), where mustard oil cake and BC replaced soybean meal (SBM), whereas T1 recorded the highest cost (7.68 Tk. or $0.072/egg) using SBM.

conclusionMustard meal, rapeseed meal and BC are effective SBM substitutes in layer diets without any adverse effect on egg quality or production. These findings highlight the potential of mustard meal, rapeseed meal and BC as quality protein sources in commercial layer nutrition.

Indexed as

ChickensGastrointestinal MicrobiomeOvumAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsBrassica rapaDietDietary SupplementsFemaleGlycine maxMustard PlantNigella sativaRandom Allocationcost‐effectivenessegg qualitygut microfloralaying henpoultry nutritionprotein source

Identifiers

PMID41811204
PMCPMC12978149

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.