Evidence map›Paper›PMID 41810939›Full record

ArticleMicrobiology spectrum2026

Comparative evaluation of viability PCR reagents highlights the superior efficacy of PMAxx azo dye for bacterial and viral viability discrimination using real-time and digital PCR.

Tiwawan Hongsibsam, Jarunee Prasertsopon, Pilaiwan Siripurkpong, Nattamon Niyomdecha

Abstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tiwawan HongsibsamGraduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, Thailand.
Jarunee PrasertsoponCenter for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, Thailand.
Pilaiwan SiripurkpongDepartment of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, Thailand.
Nattamon NiyomdechaDepartment of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, Thailand.ORCID 0000-0002-5364-6716

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viability PCR (vPCR) distinguishes viable from nonviable microorganisms by suppressing amplification from structurally compromised cells or virions. This study compared three viability reagents-ethidium monoazide (EMA), propidium monoazide derivative (PMAxx), and platinum (IV) chloride (PtCl₄)-for differentiating viable and heat-inactivated

Indexed as

Microbial ViabilityPolymerase Chain ReactionPropidiumReal-Time Polymerase Chain ReactionAzidesEscherichia coliHumansIndicators and ReagentsStaphylococcus aureus8-azidoethidiumAzidesIndicators and ReagentsPropidiumpropidium monoazidedigital PCREMApathogen detectionPMAxxPtCl₄viability PCR (vPCR)

Identifiers

PMID41810939
PMCPMC13055348

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.