Evidence map›Paper›PMID 41810929›Full record

ArticleAnalytical cellular pathology (Amsterdam)2026

PYCR1 Downregulation Induces Autophagy Dependent Apoptosis Through Inhibiting PI3K/AKT/mTOR Axis in Human Hepatocellular Carcinoma Cells.

Junli Zhang, Yachao Hou, Xinxin Jin, Biao Gu, Wenjuan Wu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical cellular pathology (Amsterdam), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Junli ZhangDepartment of Blood Transfusion, Bengbu Third People's Hospital Affiliated to Bengbu Medical University, Bengbu, 233030, Anhui, China.ORCID https://orcid.org/0009-0008-5581-0429
Yachao HouBengbu Medical University Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, Bengbu Medical University, Bengbu, 233030, Anhui, China, bbmc.edu.cn.
Xinxin JinBengbu Medical University Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, Bengbu Medical University, Bengbu, 233030, Anhui, China, bbmc.edu.cn.
Biao GuDepartment of Blood Transfusion, Bengbu Third People's Hospital Affiliated to Bengbu Medical University, Bengbu, 233030, Anhui, China.
Wenjuan WuDepartment of Biochemistry and Molecular Biology, School of Laboratory Medicine, Bengbu Medical University, Bengbu, 233030, Anhui, China, bbmc.edu.cn.ORCID https://orcid.org/0009-0009-5614-6803

Funding

Anhui Province Traditional Chinese Medicine Inheritance and Innovation Research Project 2025CCCX097Bengbu Central Hospital Research Project 2025bbsy16Bengbu Health Research General Project BBWK2024A207
6 · The paper itself

Abstract

backgroundHepatocellular carcinoma (HCC) is a global malignant tumor type. Pyrroline-5-carboxylate reductase 1 (PYCR1) is a metabolic enzyme that exhibits pro-tumor properties in cancer progression. However, the exact molecular mechanism of PYCR1 in HCC progression is still unclear.

methodsCCK8, EdU, and Transwell assays were used to measure the proliferation, migration, and invasion of HCC cells, respectively. Immunostaining and flow cytometry were used to detect cellular autophagy and apoptosis.

resultsThe downregulation of PYCR1 can inhibit the survival, proliferation, migration, and invasion of HCC cells. At the same time, downregulation of PYCR1 induces autophagy and subsequently activates cell apoptosis. Therefore, we pretreated HCC cells with mTOR activators or inhibitors to inhibit or promote autophagy, leading to an inhibition or an increase in apoptosis. Simultaneously, the PI3K activators or inhibitors to activate or inhibit the PI3K/AKT/mTOR pathway also lead to inhibition or activation of autophagy and apoptosis.

conclusionThe downregulation of PYCR1 induces autophagy-dependent apoptosis in HCC cells by inhibiting the PI3K/AKT/mTOR pathway, revealing a novel mechanistic link in HCC pathophysiology.

Indexed as

ApoptosisAutophagyCarcinoma, HepatocellularDown-RegulationLiver NeoplasmsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktPyrroline Carboxylate ReductasesSignal TransductionTOR Serine-Threonine KinasesCell Line, TumorCell MovementCell Proliferationdelta-1-Pyrroline-5-Carboxylate ReductaseHumansdelta-1-Pyrroline-5-Carboxylate ReductaseMTOR protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktPyrroline Carboxylate ReductasesTOR Serine-Threonine Kinasesapoptosisautophagyhepatocellular carcinomapyrroline-5-carboxylate reductase 1

Identifiers

PMID41810929
PMCPMC13580805

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