Evidence map›Paper›PMID 41809593›Full record

ReviewFrontiers in microbiology2026

Diagnostic methods for protozoan diseases: a review focused on leishmaniasis, Chagas disease and malaria.

Ada da Silva Matos, Rodrigo Nunes Rodrigues-da-Silva, Thais Stelzer Toledo, Laura Sant'Anna Ataides, Natália Debize da Motta, Cinthia Magalhães Rodolphi, Isabela Ferreira Soares, Francini Neves Ribeiro, Ana Luiza Carneiro Alencar, Fernanda de Moraes Maia and 2 more

Abstract readReview
In one paragraph

Review in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ada da Silva MatosImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Rodrigo Nunes Rodrigues-da-SilvaHantaviruses and Rickettsioses Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil.
Thais Stelzer ToledoImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Laura Sant'Anna AtaidesHantaviruses and Rickettsioses Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil.
Natália Debize da MottaImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Cinthia Magalhães RodolphiImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Isabela Ferreira SoaresImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Francini Neves RibeiroImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Ana Luiza Carneiro AlencarImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Fernanda de Moraes MaiaHantaviruses and Rickettsioses Laboratory, IOC, Fiocruz, Rio de Janeiro, Brazil.
Josué da Costa Lima-JuniorImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
Fernanda Nazaré MorgadoImmunoparasitology Laboratory, Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protozoan diseases remain a serious public health challenge, particularly in countries such as Brazil, whose continental dimensions and diverse ecological settings allow for multiple transmission cycles, involving a wide range of vectors, reservoirs, intermediate and definitive hosts, suitable habitats, and a complex socioeconomic context that increases exposure to various diseases due to social vulnerability. Consequently, diseases such as malaria, leishmaniasis, and Chagas disease are highly prevalent in Brazil, affecting a significant portion of the population, especially in regions marked by greater social inequality. In this context, this study aims to present the epidemiological landscape of these diseases and discuss the role of immunological and molecular diagnostic tools, as well as the fundamental concepts that are essential for evaluating these diagnostic approaches. Overall, this review provides a detailed summary of established diagnostic approaches of these diseases and emphasizes the integration of clinical and epidemiological information with the application of sensitive and specific diagnostic techniques aimed at promoting early detection, monitoring, and control of infections in endemic areas, highlighting the important role of diagnosis as a strategic tool in public health.

Indexed as

Chagas diseasediagnosisleishmaniasismalariaprotozoanreview

Identifiers

PMID41809593
PMCPMC12967969

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.