ArticleCellular & molecular biology letters2026
miR-223-3p improves macrophage polarization balance and mitochondrial metabolism to ameliorate uveitis by orchestrating P65
Article in Cellular & molecular biology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- From pathogenic to reparative: Context-Dependent function of extracellular vesicles in osteoarthritis.Bioactive materials · 2027Review
- Probiotics and Extracellular Vesicles as Redox Modulators in Wound Healing: From Microbial Therapeutics to Engineered Nanotherapeutic Strategies.Antioxidants (Basel, Switzerland) · 2026Review
- IL-Mediated Macrophage Polarization Axis: From Inflammatory Positive Feedback Loop to Precision Biomarkers and Therapeutic Targets for Allergic Rhinitis-A Narrative Review.Journal of asthma and allergy · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
Abstract
backgroundUveitis is an autoimmune disease characterized by iris, ciliary muscle, and choroid inflammation. miR-223-3p, an anti-inflammatory microRNA, can regulate the expression of inflammatory genes involved in the disease process. However, the role and potential mechanism of miR-223-3p against uveitis remain unclear.
methodsSTRING website prediction, molecular docking, and co-IP experiments were performed to verify whether there was an interaction between RBPJ-HDAC1, HDAC1-P65, and P65-ARG1. Based on ex vivo and in vivo experiments, we detected NF-κB P65lys310 acetylation, P65 nuclear translocation, and the level of M1/M2 macrophage polarization. In addition, we also determined the levels of mitochondrial pressure and calcium flux under different conditions. Regulation of NF-κB P65lys310 acetylation via the RBPJ/HDAC1 axis affects macrophage polarization and mitochondrial function.
resultsWe first found reduced miR-223-3p expression, elevated RBPJ and total acetylation levels, and an imbalance in macrophage polarization in peripheral blood monocyte-derived macrophages from patients with uveitis. Co-IP experiments supported the interaction between RBPJ and HDAC1, and HDAC1, as a key deacetylase, could inhibit NF-κB P65lys310 acetylation. Notably, overactivation of NF-κB P65lys310 acetylation levels in uveitis leads to elevated polarization of M1 macrophages and mitochondrial dysfunction. miR-223-3p can attenuate NF-κB P65lys310 acetylation and P65 nuclear translocation levels through the RBPJ/HDAC1 axis in uveitis, effectively reducing pro-inflammatory macrophage levels and mitigating mitochondrial damage, thereby reducing ocular inflammation and positively regulating the intraocular microenvironment in uveitis.
conclusionmiR-223-3p can inhibit P65lys310 acetylation and enhance mitochondrial function to improve M1/M2 macrophage polarization balance to ameliorate uveitis through RBPJ/HDAC1 axis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.