Evidence map›Paper›PMID 41806308›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Genome-Wide Association Study Reveals Insect Genetics and Microbial Symbiont Effects on Susceptibility of Diaphorina citri to the Citrus Greening Pathogen, Candidatus Liberibacter Asiaticus.

Kai Liu, Qingcui He, Zeyue Lin, Shixuan Huang, Zichun Zhong, Pingyang Zhu, Mengge Gao, Luyao Zhao, Han Jin, Guiting Wu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Kai LiuKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Qingcui HeKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Zeyue LinKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Shixuan HuangState Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou, Guangdong, P. R. China.
Zichun ZhongState Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou, Guangdong, P. R. China.
Pingyang ZhuCollege of Life Sciences, Zhejiang Normal University, Jinhua, P. R. China.
Mengge GaoKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Luyao ZhaoKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Han JinKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Guiting WuKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Gurr M GeoffGulbali Institute, Charles Sturt University, Orange, New South Wales, Australia.
Qunxin HanKey Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, P. R. China.
Rui PangState Key Laboratory of Green Pesticide, College of Plant Protection, South China Agricultural University, Guangzhou, Guangdong, P. R. China.ORCID https://orcid.org/0000-0001-9300-6142

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2025A1515012591Guangdong Province Key Discipline Research Capacity Enhancement Project 2022ZDJS020Guangdong Provincial Universities Characteristic Innovation Project 2023KTSCX046Guangzhou Science and Technology Plan Project 2024A04J4995National Natural Science Foundation of China 32001903
6 · The paper itself

Abstract

Insect-vectored pathogens pose a significant threat to global agriculture. The colonization efficiency of pathogens in vectors plays a central role in these pathosystems, yet studies of the factors that affect this aspect are limited. This study investigates the genetic and microbial symbiont factors influencing the susceptibility of Diaphorina citri to Candidatus Liberibacter asiaticus (CLas), the pathogen causing citrus greening disease (huanglongbing). Through a microbiome Genome Wide Association Study (mGWAS) based on 16S amplicon sequencing and genomic resequencing of 120 D. citri individuals from six populations, we identified 79 SNPs significantly associated with the relative abundance of CLas within insects. Additionally, some of these SNPs were also associated with the relative abundance of Candidatus Profftella armature, a key endosymbiont of D. citri. SNPs in the regulatory region of gene Dcitr04g11610.1 led to its overexpression in CLas-susceptible D. citri, and CLas infection further elevated its expression. Conversely, RNAi knockdown of Dcitr04g11610.1 reduced CLas infection rates and abundance, accompanied by increased abundance of Profftella. Phylogenetic analysis revealed Dcitr04g11610.1's high homology to Major Facilitator Superfamily-type transporter SLC18B1 proteins, suggesting a role in CLas polyamine utilization. These findings highlight the importance and potential interplay of insect genetics and symbiotic microbiota in insect-vectored plant pathogen systems.

Indexed as

CitrusHemipteraLiberibacterPlant DiseasesRhizobiaceaeSymbiosisAnimalsGenome-Wide Association StudyMicrobiotaPolymorphism, Single Nucleotidecitrus greening pathogendiaphorina citrigeneticssusceptibilitysymbiotic microbiota

Identifiers

PMID41806308
PMCPMC13205867

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.