A cloud-based solution for managing next-generation sequencing data to comprehend forensic population statistics.

Next-generation sequencing (NGS) technology has revolutionized forensic DNA analysis by increasing sensitivity to detect low amounts of DNA, expanding throughput capacity, allowing multiplexed marker panels, and improving sequencing resolution. However, the diversity of marker types and the complexity of report formatting have made managing and utilizing genetic statistics for large volumes of NGS data challenging. To address these challenges, advancements in bioinformatics tools and data management systems are essential. Implementing a public database can facilitate more effective popular statistics, ultimately enhancing the application of NGS in forensic investigations. We introduce “diversID,” a forensic DNA database ( https://www.diversid.org ) focused on autosomal STRs (short tandem repeats), X- and Y-chromosomal STRs, and identity SNPs (single nucleotide polymorphisms). The database software provides comprehensive population statistics, including allele frequencies, Hardy-Weinberg equilibrium, heterozygosity, and more. Making use of cloud computing, diversID simplifies data upload and download processes to improve user convenience while maintaining the highest data quality through stringent filtering criteria. To further enrich the database’s value, we invite researchers to encourage global collaboration. We believe that collective efforts will continually refine and advance this invaluable resource, thereby benefiting the field of forensic DNA analysis.