Evidence map›Paper›PMID 41805370›Full record

ArticleThe journal of physical chemistry. B2026

A Highly Sensitive Water-Soluble Donor-Acceptor Dye for Early-Stage Amyloid Aggregation Kinetics.

Giorgio Scattolini, Carlos Enrique Torres-Méndez, Dylan Valli, Mikołaj Ignacy Kuska, Nidhi Kaul, Leif Hammarström, Haining Tian, Michał Maj

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of physical chemistry. B, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Giorgio ScattoliniDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0000-0003-1300-1777
Carlos Enrique Torres-MéndezDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0000-0003-2841-7724
Dylan ValliDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0009-0005-6060-4169
Mikołaj Ignacy KuskaDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.
Nidhi KaulDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0000-0002-4095-0487
Leif HammarströmDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0000-0002-9933-9084
Haining TianDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0000-0001-6897-2808
Michał MajDepartment of Chemistry - Ångström Laboratory, Uppsala University, Box 523, 751 20 Uppsala, Sweden.ORCID 0000-0003-1567-9514

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein aggregation into amyloid fibrils underlies numerous human diseases, yet the most widely used fluorescent probe, Thioflavin T (ThT), offers an incomplete picture of the process and fails to detect certain fibril structures. Here, we introduce and characterize the photophysical properties of DANIR-2b(2OH), a water-soluble push-pull dye that overcomes these limitations. It successfully binds early prefibrillar aggregates and small fibrils of the human Islet Amyloid Polypeptide that elude detection by ThT, which we confirm by time-resolved cryo-electron microscopy of aliquots taken during the kinetic assays. We further demonstrate that DANIR-2b(2OH) can also track the aggregation of other amyloid proteins, such as insulin and Aβ

Indexed as

AmyloidFluorescent DyesIslet Amyloid PolypeptideAmyloid beta-PeptidesBenzothiazolesCryoelectron MicroscopyHumansKineticsProtein AggregatesSolubilitySpectrometry, FluorescenceWaterAmyloidAmyloid beta-PeptidesBenzothiazolesFluorescent DyesIslet Amyloid PolypeptideProtein Aggregatesthioflavin TWater

Identifiers

PMID41805370
PMCPMC13006956

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.