ArticleHealth science reports2026
Quantitative Histomorphometric Analysis of Collagen Bundles in Masson's Trichrome Stained Rat (
Article in Health science reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- A Three-Dimensional Hyaluronic Acid-Based Scaffold Model for Electroporation-Enhanced Resveratrol Therapy in Triple-Negative Breast Cancer.Cell biochemistry and biophysics · 2026Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and Aims: While Masson's Trichrome is widely used for collagen visualization, its qualitative nature limits reproducibility and statistical comparison. A methodological gap persists in the quantitative assessment of collagen in Methods: Formalin-fixed, paraffin-embedded skin tissue from Wistar rats was stained with Masson's Trichrome. Digital images were analyzed using ImageJ. The protocol involved creating custom stain vectors for precise color deconvolution, separating the image into blue-green (collagen) and red-pink (non-collagenous) channels. Quantitative features, including area, intensity, skewness, and kurtosis, were extracted from the thresholded regions of each stain. Results: Analysis of 168 images showed that the blue-green (collagen) stained area was significantly larger than the red-pink area ( Conclusion: This work provides a validated framework for the objective and reproducible quantification of collagen in rat skin. By bridging traditional histology with computational analysis, this protocol offers a robust tool for future studies on collagen dynamics in skin pathologies and wound healing, with potential applications in evaluating therapeutic strategies.
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