Evidence map›Paper›PMID 41803924›Full record

ReviewAnimal microbiome2026

Towards standardization in pig microbiome research based on a comprehensive twenty-year review.

Samuel Onyilokwu Enokela, Timur Yergaliyev, Krzysztof Flisikowski, Stéphanie Céline Hornburg, Henry Reyer, Jens Tetens, Klaus Wimmers, Jürgen Zentek, Amélia Camarinha-Silva

Abstract readReview
In one paragraph

Review in Animal microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Samuel Onyilokwu EnokelaInstitute of Animal Science, University of Hohenheim, Emil-Wolff-Str. 10, Stuttgart, Germany.
Timur YergaliyevInstitute of Animal Science, University of Hohenheim, Emil-Wolff-Str. 10, Stuttgart, Germany.
Krzysztof FlisikowskiInfection Pathogenesis, Technical University of Munich, Liesel-Beckmann-Straße 1, Freising, Germany.
Stéphanie Céline HornburgInstitute for Animal Nutrition and Physiology, Christian-Albrechts University, Hermann-Rodewald-Straße 9, Kiel, Germany.
Henry ReyerResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, Dummerstorf, Germany.
Jens TetensDepartment of Functional Breeding, Georg-August University, Burckhardtweg 2, Göttingen, Germany.
Klaus WimmersResearch Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, Dummerstorf, Germany.
Jürgen ZentekInstitute of Animal Nutrition, Freie Universität-Berlin, Königin-Luise-Str. 49, Berlin, Germany.
Amélia Camarinha-SilvaInstitute of Animal Science, University of Hohenheim, Emil-Wolff-Str. 10, Stuttgart, Germany. amelia.silva@uni-hohenheim.de.

Funding

German Federal Ministry of Food and Agriculture 28N-2-051-01
6 · The paper itself

Abstract

The pig microbiome plays a crucial role in animal health, productivity, and meat quality. This comprehensive review analyzed 438 publications on pig microbiome studies from 2003 to 2023, focusing on experimental methodologies, bioinformatic tools, sequencing approaches, and metadata reporting practices. It critically analyzes the variability introduced by different DNA extraction methods, sequencing platforms, and bioinformatics pipelines, emphasizing their impacts on reproducibility and data comparability. We identify critical gaps in metadata reporting, particularly in DNA extraction protocols and bioinformatic workflows. To address these issues, we propose a standardized metadata template for pig microbiome studies, integrating Genomic Standards Consortium guidelines with project-specific criteria. This review provides a framework for developing standardized protocols in pig microbiome research, aiming to improve reproducibility and facilitate cross-study comparisons.

Indexed as

MetadataMicrobiomePigStandardization

Identifiers

PMID41803924
PMCPMC13063546

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.