Evidence map›Paper›PMID 41803797›Full record

ArticleBMC complementary medicine and therapies2026

Guineenoside B a triterpenoid saponin from Piper guineense (Piperaceae) triggers apoptosis and PI3K/Akt/mTOR inactivation and inhibits metastasis.

Eutrophe Le Doux Kamto, Anne Offermann, Olivier Placide Noté, Joséphine Ngo Mbing, Mohamed Haddad, Dieudonné Emmanuel Pegnyemb, Dieudonné Njamen, Susan L Bonnet, Sven Perner, Stephane Zingue

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Article in BMC complementary medicine and therapies, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Eutrophe Le Doux KamtoDepartment of Organic Chemistry, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon. lekamto@gmail.com.
Anne OffermannInstitute of Pathology, University Hospital Schleswig-Holstein, Luebeck, 23538, Germany.
Olivier Placide NotéDepartment of Organic Chemistry, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon.
Joséphine Ngo MbingDepartment of Organic Chemistry, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon.
Mohamed HaddadUMR 152 Pharma Dev, Université de Toulouse, IRD, UPS, Toulouse, France.
Dieudonné Emmanuel PegnyembDepartment of Organic Chemistry, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon.
Dieudonné NjamenDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaounde, Cameroon.
Susan L BonnetDepartment of Chemistry, University of the Free State, 205 Nelson Mandela Avenue, Bloemfontein, 9301, South Africa.
Sven PernerInstitute of Pathology, University Hospital Schleswig-Holstein, Luebeck, 23538, Germany.
Stephane ZingueInstitute of Pathology, University Hospital Schleswig-Holstein, Luebeck, 23538, Germany. stephane.zingue@fmsb-uy1.cm.

Funding

Organization for the Prohibition of Chemical Weapons OPCW 188/22 1/3 INS
6 · The paper itself

Abstract

backgroundOver 1.4 million new cases of prostate cancer (PCa) and 375,304 deaths were recorded in 2020. Piper guineense Schum and Thonn is used to treat various diseases including cancer. Three triterpenoid saponins namely guineenosides (Guin) A, B & C were recently isolated from this plant. This study marks the initial documentation of the anticancer potential of these compounds.

methodsAnti-cell growth (MTT), proliferation (CCK-8), clonogenic assays were evaluated. Furthermore, the most potent compound (Guin-B) was investigated for its potential to induce apoptosis, inhibit cell migration/invasion as well as cell adhesion. Some key proteins in cell proliferation and migration were evaluated.

resultsOut of the 3 saponins isolated from P. guineense, Guin-B exhibited significant and concentration-dependent inhibition of PC3 (IC50 =10 µg/mL or ~ 4.82 × 10− 6 µM) and LNCaP (IC50 = 8 µg/mL or 3.86 × 10− 6 µM) cell growth and proliferation as well as a potent inhibition (p < 0.001) of PC3 cell clone formation. Guin-B induced a potent (near 50%) increment of apoptotic cell population in PC3 and LNCaP cells at both 5 and 10 µg/mL; which was accompanied by a significant increase in caspase-3 activity. Guin-B induced a potent inhibition of PC3 cell migration and invasion and an increase in adherence of PC3 cells to the extracellular collagen and fibronectin matrix. A decrease in the expression of pAKT, AKT, mTOR and VEGF proteins was noticed following the treatment with Guin-B.

conclusionGuineenosides B triggers apoptosis via mitochondrial pathway and PI3K/Akt/mTOR inactivation and inhibits metastasis at a low concentration, thus encouraging future investigation of this saponin.

Indexed as

Antineoplastic Agents, PhytogenicApoptosisPiperPlant ExtractsProstatic NeoplasmsSaponinsTriterpenesCell Line, TumorCell MovementCell ProliferationHumansMalePC-3 CellsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesAntineoplastic Agents, PhytogenicMTOR protein, humanPhosphatidylinositol 3-KinasesPlant ExtractsProto-Oncogene Proteins c-aktSaponinsTOR Serine-Threonine KinasesTriterpenesApoptosisMetastasisPiper guineenseProstate cancerTriterpenoid saponin

Identifiers

PMID41803797
PMCPMC13185189

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.