Evidence map›Paper›PMID 41803700›Full record

ArticleBMC plant biology2026

Mechanism of tobacco hairy root development and functional study of the HSF gene family based on comparative transcriptomics.

Xiaozong Wu, Zhitao Qi, Lei Wang, Yaolan Zhuang, Yixuan Xue, Haoyu Yan, Jifan He, Peilin Li, Zhiwen Zhu, Guiliang Tang and 2 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Xiaozong WuInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Zhitao QiInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Lei WangChina Tobacco Yunnan Industrial CO., LTD. , Kunming, 650000, China.
Yaolan ZhuangInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Yixuan XueInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Haoyu YanInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Jifan HeInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Peilin LiInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Zhiwen ZhuInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China.
Guiliang TangDepartment of Biological Sciences, Michigan Technological University, Houghton, MI, USA.
Meng LiInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China. limengjeff@126.com.
Chaonan ShiInternational Joint Research Laboratory for Utilization of Plant Functional Components, College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450001, China. 2023055@zzuli.edu.cn.

Funding

Doctoral fund project of Zhengzhou University of Light Industry, China Project No.: 2024BSJJ011Henan Province Science and Technology Research Project 242102110334Henan Province Science and Technology Research Project 252102110270Henan Provincial Natural Science Foundation Project No.: 252300420221
6 · The paper itself

Abstract

Hairy roots induced by Agrobacterium rhizogenes exhibit unique phenotypic traits such as rapid proliferation, hormone-independent growth. To further elucidate the transcriptomic differences between hairy roots and the roots, stems, and leaves of normal plants, as well as their potential mechanisms of formation, this study conducted a comparative transcriptomic analysis. The results indicated that differentially expressed genes (DEGs) were significantly enriched in pathways related to energy metabolism, stress response, and secondary metabolism. Then weighted gene co-expression network analysis (WGCNA) analysis identified two key co-expression modules. Moreover, among the differentially expressed transcription factors (TFs), the HSF (heat shock transcription factor) family showed the most significant enrichment. Further analysis of 17 HSFs revealed that all of them were significantly correlated with plant hormones. Among these, two were specifically expressed in hairy roots, and nine exhibited both constitutive high expression and responsiveness to salicylic acid (SA), melatonin and drought treatments. These results establish an HSF-mediated regulatory network, offering valuable theoretical insights and potential targets for breeding stress-resistant germplasm with enhanced root systems, as well as for the synthetic biology-based engineering of hairy roots.

Indexed as

Heat Shock Transcription FactorsNicotianaPlant ProteinsPlant RootsTranscriptomeAgrobacteriumGene Expression ProfilingGene Expression Regulation, PlantGene Regulatory NetworksMultigene FamilyHeat Shock Transcription FactorsPlant ProteinsDifferentially expressed genes (DEGs)Gene expression patternGene regulatory networkHeat shock transcription factors (HSFs) familyTabacco hairy roots

Identifiers

PMID41803700
PMCPMC13085611

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.