ArticleApplied microbiology and biotechnology2026
Growth and protein content of Cupriavidus necator on organic acids derived from fermented grey starch.
Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Grey starch (GS) is a byproduct of potato processing and is conventionally valorised as low-grade animal feed or for biogas generation. In this manuscript, we systematically investigate the mixed culture fermentation of GS to organic acids and their subsequent aerobic upgrading to microbial protein via Cupriavidus necator. We focused on protein content, growth kinetics, biomass yield on individual, and mixed organic acids (C1-C6), using both synthetic media and real fermented effluents. High-throughput cultivation in microtiter plates was employed to evaluate performance across both straight-chain forms and isoforms of the acids. C. necator demonstrated growth on all tested individual substrates, although with distinct individual behaviour. Lactate, butyrate, and hexanoate supported the highest biomass yields, reaching up to 0.24 gCDW/gCOD (grammes of cell dry weight per grammes of COD fed). Lactate enabled the highest specific growth rate (0.6 h
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