Evidence map›Paper›PMID 41803252›Full record

ArticleScientific reports2026

Morpho-biochemical and molecular identification of Bacillus licheniformis and Bacillus cereus isolates from sorghum (Sorghum bicolor L.) rhizosphere.

Angeline G Jurry, Jyoti Prakash Sahoo, Siddhartha Shankar Sharma, Jannila Praveena, Shaikh Nausad Hossain, Nandini Sahu, Subhadarsini Pradhan, Priyanka Nayak, Swarnalata Tripathy, Dhaarani Vijayakumar and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Angeline G JurryDepartment of Biotechnology, C.V. Raman Global University, Bhubaneswar, 752054, India.
Jyoti Prakash SahooDepartment of Biotechnology, C.V. Raman Global University, Bhubaneswar, 752054, India. jyotiprakashsahoo2010@gmail.com.
Siddhartha Shankar SharmaFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Jannila PraveenaFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Shaikh Nausad HossainRegional Institute of Biotechnology, Bhubaneswar, 751016, India.
Nandini SahuFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Subhadarsini PradhanFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Priyanka NayakFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Swarnalata TripathyFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Dhaarani VijayakumarFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Sashanka Sekhar DashFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Biswajit JenaFaculty of Agriculture and Allied Sciences, C.V. Raman Global University, Bhubaneswar, 752054, India.
Pranaya PradhanNICRA Project, Krishi Vigyan Kendra, Odisha University of Agriculture and Technology, Puri, 752014, India.
Samikshya Sankalini PradhanDepartment of Plant Pathology, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study isolated, characterized, and identified rhizosphere-associated soil bacteria from sorghum-cultivated soils in Bhubaneswar, India, using serial dilution and spread plate techniques on Nutrient Agar. Morphological and biochemical analyses (Gram staining, catalase, oxidase, MR, and indole tests) identified 13 bacterial isolates, predominantly Gram-positive rods (Bacillus, Paenibacillus) and cocci (Staphylococcus, Micrococcus), with one Gram-negative isolate. Molecular characterization involved 16S rRNA gene amplification (~ 1500 bp), sanger sequencing, and phylogenetic analysis. BLASTN confirmed AG3 as Bacillus licheniformis (99.37% identity) (GenBank: PV590072), and AG11 as Bacillus cereus (100% identity) (GenBank: PV590099). The phylogenetic tress analysis of bacterial isolates AG3 and AG11 revealed distinct evolutionary rate variations. For AG3, site-specific rates ranged from 0.07 to 3.64 substitutions/site (nucleotide frequencies: A = 24.50%, T/U = 19.99%, C = 24.70%, G = 30.81%), while AG11 exhibited more uniform rates ranged from 0.90 to 1.10 substitutions/site (nucleotide frequencies: A = 25.79%, T/U = 20.86%, C = 22.71%, G = 30.64%). Maximum likelihood trees (log-likelihood: - 4077.853 for AG3, - 1928.562 for AG11) with 1,531 (AG3) and 1,402 (AG11) aligned positions resolved their phylogenetic relationships. This study provides valuable insights into the diversity and evolutionary dynamics of rhizosphere-associated Bacillus spp. in sorghum-cultivated soils, contributing to a better understanding of their ecological significance and potential application in sustainable agriculture and plant growth promotion.

Indexed as

Bacillus cereusBacillus licheniformisRhizosphereSorghumPhylogenyRNA, Ribosomal, 16SSoil MicrobiologyRNA, Ribosomal, 16SGenotypic profilingGram-stainingPhenotypic characterizationRhizosphere microbiomeSorghum

Identifiers

PMID41803252
PMCPMC12992546

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.