Evidence map›Paper›PMID 41802127›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Single-Cell Transcriptomics Reveals FLS2-Dependent Hypoxia Signaling and ERF13-Mediated Transcription During flg22-Triggered Immunity.

Yaping Zhou, Aizhi Qin, Mengfan Li, Qianli Zhao, Luyao Kong, Lulu Yan, Chunyang Li, Hao Liu, Yinpeng Zhang, Jiani Long and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Functional Characterization ofPlants (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Yaping ZhouNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Aizhi QinNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Mengfan LiNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Qianli ZhaoNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Luyao KongNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Lulu YanNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Chunyang LiNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Hao LiuNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Yinpeng ZhangNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Jiani LongNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Mengyu LiaoNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Mengmeng ZhouNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Xiaoli FanNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Baozhen WangNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Wenxuan KangNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Shui WangCollege of Life Sciences, Shanghai Normal University, Shanghai, China.
Zhixin LiuNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.
Xuwu SunNational Key Laboratory of Cotton Bio-breeding and Integrated Utilization, State Key Laboratory of Crop Stress Adaptation and Improvement, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 85 Minglun Street, Kaifeng, Henan, China.ORCID https://orcid.org/0000-0001-8462-6948

Funding

Key Research Projects of Colleges and Universities in Henan Province 23A180004National Key Research and Development Program of China 2022YFD1200300State Key Laboratory of Cotton Bio-breeding and Integrated Utilization Open Fund CB2024A22,CB2024A19
6 · The paper itself

Abstract

The flagellin peptide flg22 activates FLAGELLIN-SENSING 2 (FLS2)-mediated immunity in Arabidopsis, leading to growth inhibition and oxidative burst. While these responses are well-studied, their cell-type-specific regulation remains poorly understood. Using single-cell RNA sequencing, genetics, and phenotyping, we systematically mapped flg22-induced responses. flg22 suppressed growth and elevated reactive oxygen species (ROS) in wild-type, but not in fls2 mutants. Epidermal (EP_3) and mesophyll (MPC_2) cells showed FLS2-dependent transcriptional reprogramming. Pseudotime analysis revealed developmental trajectories toward immune-activated states. flg22 also induced a hypoxia-like response; hypoxic signaling mutants (ate1, prt6, zpr2) showed reduced flg22 sensitivity, indicating crosstalk between immune and hypoxia pathways. ERF13 was identified as a central regulator: erf13 mutants impaired flg22-triggered ROS and growth inhibition but enhanced effector-triggered immunity (ETI), while overexpressors showed stronger pattern-triggered immunity (PTI). flg22 altered ploidy and cell-cycle gene expression in WT, which was stabilized in ate1 and erf13 mutants. Cell-cycle mutants sim smr and e2fabc enhanced flg22 responses, whereas cpr5 was less sensitive. Thus, immune, hypoxia, and ROS signals converge via ERF13 to balance immunity and growth, providing a single-cell view of spatial immune organization and stress adaptation.

Indexed as

ArabidopsisArabidopsis ProteinsFlagellinPlant ImmunityProtein KinasesTranscriptomeGene Expression Regulation, PlantReactive Oxygen SpeciesSignal TransductionSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisArabidopsis ProteinsFlagellinFLS2 protein, ArabidopsisProtein KinasesReactive Oxygen SpeciesERF13FLS2Hypoxic signalingScRNA‐seqTranscription factor network

Identifiers

PMID41802127
PMCPMC13185882

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.