ArticleSoft matter2026
Quantification of cholesterol incorporation in giant unilamellar vesicles produced by a modified cDICE method.
Article in Soft matter, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Cholesterol is an essential component of eukaryotic cell membranes, influencing membrane packing, fluidity, and domain formation. Replicating these properties in model membranes is critical for reconstitution studies, but common emulsion-based methods for producing giant unilamellar vesicles (GUVs) fail to incorporate cholesterol efficiently. Here, we use methyl-β-cyclodextrin-cholesterol (MβCD-CL) complexes to deliver cholesterol into GUVs produced by the emulsion droplet interface crossing encapsulation (eDICE) method and demonstrate a convenient way to quantify the degree of cholesterol incorporation using fluorescent membrane biosensors. Spectral imaging of NR12A as well as fluorescence lifetime imaging of Flipper-TR revealed dose-dependent increases in cholesterol content for DOPC GUVs upon MβCD-CL addition, consistent with increased membrane order. By calibrating these effects against GUVs with defined cholesterol contents prepared
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