SynthesisVeterinary medicine and science2026
Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review.
Synthesis in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Bacterial Bovine Mastitis: A Systematic Review.Veterinary medicine and science · 2026Pooled it
- Comparative Evaluation of 16S rRNA Target Regions Using PCR High-Resolution Melt Curve Analysis for Differentiation of Bacteria Associated with Bovine Mastitis.Microorganisms · 2026Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine bacterial mastitis is an inflammation of cows' udders by bacterial infection, leading to significant economic, animal welfare and public health consequences. Conventional diagnostic tests are expensive, time-consuming, and require specialised laboratories, making them unsuitable for on-farm surveillance and timely interventions. As an alternative, loop-mediated isothermal amplification (LAMP) has gained attention as a rapid, cost-effective and sensitive molecular diagnostic. LAMP amplifies DNA at a constant temperature, offering a simple and efficient method for bacterial detection. This review compiles studies on LAMP assays developed to detect major mastitis-causing bacteria. Thus, a total of 18 LAMP assays were identified using PRISMA guidelines. Colourimetric and turbidity-based LAMP assays were most frequently used. Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis and Escherichia coli were the most common bacteria detected. While LAMP demonstrates high sensitivity and specificity in laboratory settings, its efficacy in field conditions remains uncharacterised. Nevertheless, the simplicity of sample preparation, resistance to inhibitors, short turnaround time, ease of running and simple-to-interpret results make LAMP a promising method for on-farm diagnosis. Overall, this review highlights the current state of LAMP developed for bovine mastitis and identifies gaps for future studies.
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Registered trials
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