ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Multi-Targeting Non-Specific Genome Engineering in Bacteria.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Genome engineering plays a crucial role in the rapidly growing fields of metabolic engineering and synthetic biology. Chromosomal integration and stable expression of functional genes or large metabolic pathways necessitate the development of host-independent enabling technologies in diverse bacteria. Here, a generalizable genome engineering approach, MNGE (Multi-targeting Non-specific Genome Engineering), is developed based on the multi-targeting integrase (MTI) systems for multi-copy (at least three copies), highly random (only requiring the core TT dinucleotide) integration of metabolic genes or pathways in both Gram-positive bacteria (i.e., Streptomyces and Saccharopolyspora) and Gram-negative bacteria (i.e., Burkholderia and Chromobacterium). Using MNGE, the fungicide UK-2 BGC (41 kb) and the polyether antibiotic salinomycin BGC (106 kb) were randomly integrated into a heterologous host Streptomyces albus, significantly enhancing their fermentation levels based on chromosome position effects. Furthermore, the potent G
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